COLOCALIZATION OF BMP-7 AND BMP-2 RNAS SUGGESTS THAT THESE FACTORS COOPERATIVELY MEDIATE TISSUE INTERACTIONS DURING MURINE DEVELOPMENT
COLOCALIZATION OF BMP-7 AND BMP-2 RNAS SUGGESTS THAT THESE FACTORS COOPERATIVELY MEDIATE TISSUE INTERACTIONS DURING MURINE DEVELOPMENT
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DOI：
10.1016/0925-4773(94)00326-i
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发表时间：
1995-03-01
影响因子：
2.6
通讯作者：
ROBERTSON, EJ
中科院分区：
文献类型：
--
作者：
LYONS, KM;HOGAN, BLM;ROBERTSON, EJ
转化生长因子β（TGFβ）相关的分子的骨形态发生蛋白（BMP）的成员与整个脊椎动物发育的各种归纳过程有关。 BMP的60A子类包含至少四个脊椎动物成员，BMPS 5-8。我们已经通过库筛选和原位杂交表明，在这四个基因中，BMP 7最早表示，在胃胚胎中。此外，在整个开发过程中，BMP 7转录本都存在于不同的地点，其模式与各种电感相互作用的作用一致。最近的研究表明，与相应的同型二聚体相比，BMP 2/7异二聚体具有独特的活性。因此，我们使用原位杂交比较了BMP 2和BMP 7的表达模式。我们的结果表明，这些BMP在许多已知是电感信号来源的组织中共表达，包括偏振活性的区域和发育中的肢体的顶端外胚层脊和Notochord，从而提高了BMP 2/7的可能性。异二聚体可能介导这些组织相互作用的各个方面。我们还表明，在发育中的肠道内，BMP 2的成绩单受到背侧内胚层的限制，而声音刺猬已定位在发展中心的肠内内胚层的腹侧和中间区域。这些标记为发育中的肠道背侧/腹侧极性提供了第一个分子证据。
Members of the bone morphogenetic protein (BMP) class of transforming growth factor beta (TGF beta)-related molecules have been implicated in a variety of inductive processes throughout vertebrate development. The 60A subclass of BMPs contains at least four vertebrate members, BMPs 5-8. We have shown by library screening and in situ hybridization that of these four genes, BMP 7 is expressed earliest, in gastrulating embryos. Furthermore, BMP 7 transcripts are present at diverse sites throughout development, in a pattern consistent with a role in a variety of inductive interactions. Recent studies have shown that BMP 2/7 heterodimers have unique activities compared to the corresponding homodimers. For this reason, we compared the patterns of expression of BMP 2 and BMP 7 using in situ hybridization. Our results demonstrate that these BMPs are coexpressed in a number of tissues that are known to be the source of inductive signals, including the zone of polarizing activity and apical ectodermal ridge of the developing limb and the notochord, raising the possibility that BMP 2/7 heterodimers may mediate aspects of these tissue interactions. We also show that BMP 2 transcripts are restricted within the developing gut to dorsal endoderm, whereas sonic hedgehog has been localized to ventral and medial regions of the developing gut endoderm. These markers provide the first molecular evidence for dorsal/ventral polarity in the developing gut.