Next-generation sequencing: applications beyond genomes.
Next-generation sequencing: applications beyond genomes.
复制标题
下一代测序：基因组以外的应用。
DOI：
10.1042/bst0361091
复制
发表时间：
2008-10
影响因子：
3.9
通讯作者：
Bähler J
中科院分区：
文献类型：
--
作者：
Marguerat S;Wilhelm BT;Bähler J
30多年前DNA测序的发展对生物研究产生了深远的影响。在过去的几年中，出现了显着的技术创新，允许以前所未有的规模和速度对复杂样品进行直接和具有成本效益的测序。这些新一代技术不仅可以对静态基因组进行测序，还可以对在不同条件下表达的整个转录组进行测序。下一代测序的这些和其他强大的应用正在迅速改变基因组研究的方式。下面，我们提供了这些令人兴奋的新方法来了解基因组的性质和功能的快照。考虑到基于测序的检测可能会越来越多地取代基于微阵列的检测，我们还比较和对比了从这些不同方法获得的数据。
The development of DNA sequencing more than 30 years ago has profoundly impacted biological research. In the last couple of years, remarkable technological innovations have emerged that allow the direct and cost-effective sequencing of complex samples at unprecedented scale and speed. These next-generation technologies make it feasible to sequence not only static genomes, but also entire transcriptomes expressed under different conditions. These and other powerful applications of next-generation sequencing are rapidly revolutionizing the way genomic studies are carried out. Below, we provide a snapshot of these exciting new approaches to understanding the properties and functions of genomes. Given that sequencing-based assays may increasingly supersede microarray-based assays, we also compare and contrast data obtained from these distinct approaches.