A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan.
A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan.
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DOI：
10.1292/jvms.15-0536
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发表时间：
2016-03
期刊：
影响因子：
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通讯作者：
Mizutani T
中科院分区：
文献类型：
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作者：
Oba M;Omatsu T;Takano A;Fujita H;Sato K;Nakamoto A;Takahashi M;Takada N;Kawabata H;Ando S;Mizutani T
软蜱（Argas vespertilionis）是从日本的蝙蝠粪便中收集的。将蜱匀浆接种到 Vero 细胞后观察到细胞病变效应 (CPE)。使用下一代测序仪对从细胞上清液中提取的RNA进行测序。这些重叠群与布尼亚病毒、伊塞克库尔病毒的片段具有同一性。 L、M和S片段与伊塞克库尔病毒的同一性分别仅为77%、76%和79%。因此，我们将这种新型病毒命名为软蜱布尼亚病毒（STBV）。在系统发育树中，STBV 的 L 段与布尼亚病毒科奈罗病毒属的簇密切相关。
Soft ticks, Argas vespertilionis, were collected from feces of bats in Japan. Cytopathic effect (CPE) was observed after inoculating the homogenates of ticks to Vero cells. Sequencing of RNA extracted from the cell supernatant was performed by next generation sequencer. The contigs had identity to segments of Bunyaviruses, Issyk-Kul virus. The identities of segment L, M and S were only 77, 76 and 79% to Issyk-Kul virus, respectively. Therefore, we named this novel virus Soft tick bunyavirus (STBV). In the phylogenetic tree, segment L of STBV was closely related to a cluster consisting of the genus Nairovirus of the family Bunyaviridae.