Mycobacterium tuberculosis Rv0899 defines a family of membrane proteins widespread in nitrogen-fixing bacteria.
Mycobacterium tuberculosis Rv0899 defines a family of membrane proteins widespread in nitrogen-fixing bacteria.
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结核分枝杆菌 Rv0899 定义了广泛存在于固氮细菌中的膜蛋白家族。
DOI：
10.1002/prot.23151
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发表时间：
2011
期刊：
影响因子：
2.9
通讯作者：
Marassi,FrancescaM
中科院分区：
文献类型：
--
作者：
Marassi,FrancescaM
结核分枝杆菌膜蛋白Rv0899使细菌能够适应酸性环境。由于其C末端与细菌OmpA样结构域有很强的序列同源性，Rv0899被认为构成了一个外膜孔蛋白OFM。肺结核。然而，OmpA样结构域广泛存在于多种不同功能的细菌蛋白中。此外，Rv0899的三维结构不包含跨膜β桶，最近的证据表明它没有孔蛋白活性。相反，therv0899基因是操纵子(rv0899-rv0901)的一部分，操纵子是快速分泌氨气、中和pH和生长Fm所必需的。在酸性环境中的结核病。这些功能的实现机制尚不清楚。为了获得进一步的功能洞察，对基因组数据库进行了有针对性的搜索，以寻找序列相似于OmpA样C-末端的蛋白质。结果表明，Rv0899类蛋白在细菌中广泛存在，具有氮代谢、适应营养不良环境和/或与宿主建立共生关系的功能，并似乎形成了一个蛋白质家族。这些发现表明，我。结核Rv0899也可能协助类似的过程，并进一步支持其在氨分泌和M的作用。结核对宿主环境的适应。蛋白质2011；©2011 Wiley-Liss，Inc.
TheMycobacterium tuberculosismembrane protein Rv0899 confers adaptation of the bacterium to acidic environments. Due to strong sequence homology of its C‐terminus to bacterial OmpA‐like domains, Rv0899 has been proposed to constitute an outer membrane porin ofM. tuberculosis. However, OmpA‐like domains are widespread in a wide variety of bacterial proteins with different functions. Furthermore, the three‐dimensional structure of Rv0899 does not contain a transmembrane β‐barrel, and recent evidence demonstrates that it does not have porin activity. Instead, therv0899gene is part of an operon (rv0899‐rv0901) that is required for fast ammonia secretion, pH neutralization, and growth ofM. tuberculosisin acidic environments. The mechanism whereby these functions are accomplished is not known. To gain further functional insights, a targeted search of the genomic databases was performed for proteins with sequence similarity beyond the OmpA‐like C‐terminus. The results presented here, show that Rv0899‐like proteins are widespread in bacteria with functions in nitrogen metabolism, adaptation to nutrient poor environments, and/or establishing symbiosis with the host organism, and appear to form a protein family. These findings suggest thatM. tuberculosisRv0899 may also assist similar processes and lend further support to its role in ammonia secretion andM. tuberculosisadaptation to the host environment. Proteins 2011; © 2011 Wiley‐Liss, Inc.