A Novel Experimental Study on the Fabrication and Biological Characteristics of Canine Bone Marrow Mesenchymal Stem Cells Sheet Using Vitamin C
A Novel Experimental Study on the Fabrication and Biological Characteristics of Canine Bone Marrow Mesenchymal Stem Cells Sheet Using Vitamin C
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维生素C制备犬骨髓间充质干细胞片及其生物学特性的新实验研究
DOI：
10.1002/sca.21177
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Zhou, Nuo
中科院分区：
文献类型：
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作者：
Guo, Peng;Zeng, Jing-jing;Zhou, Nuo
本研究旨在通过维生素C构建犬骨髓间充质干细胞片层，并鉴定其生物学特性，探讨维生素C促进细胞片层形成的可能机制。本研究采用维生素C诱导骨髓间充质干细胞增殖。由此产生的细胞分泌大量胶原，从而缩短了细胞片层的构建时间。除此之外，我们还通过组织学观察、透射电镜观察、实时荧光PCR检测、免疫组化检测和成骨诱导实验等方法，鉴定了细胞片的生物学微观特征。我们能够稳定、快速地构建骨髓间充质干细胞片层，并有效地收获它，将种子细胞移植到组织工程中。这项研究表明，使用维生素C从骨髓中收获间充质干细胞片可能为骨组织再生提供一种简单实用的方法。扫描37：42-48，2015年。(c)2015 Wiley Periodicals，Inc.
The aim of this study was to fabricate canine bone marrow mesenchymal stem cell sheet through the use of vitamin C, to identify the biological characteristics of the resulting cell sheets, and to reveal the potential mechanism of cell-sheet promotion by vitamin C. This study used vitamin C to induce bone marrow mesenchymal stem cells to proliferate. The resulting cells secreted large amounts of collagen, thereby shortening the construction time of the cell-sheet layer. In addition to these aims, we identified biological microcharacteristics of the cell sheet through histological observation, transmission electron microscopy, real-time PCR detection, immunohistochemical detection, and osteogenesis-induction experiments on the cell sheet. We were able to stably and rapidly construct bone marrow mesenchymal stem cell sheet, effectively harvest it, and transfer the seed cells for tissue engineering. This study indicates that the use of vitamin C for harvesting mesenchymal stem cell sheets from bone marrow may provide an easy and practical approach for bone tissue regeneration. SCANNING 37:42-48, 2015. (c) 2015 Wiley Periodicals, Inc.