Genetic and functional relationship between Rvsp, myosin and actin in Saccharomyces cerevisiae
Genetic and functional relationship between Rvsp, myosin and actin in Saccharomyces cerevisiae
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DOI：
10.1007/s002940050397
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发表时间：
1998-10-01
期刊：
影响因子：
2.5
通讯作者：
Aigle, M
中科院分区：
文献类型：
--
作者：
Breton, AM;Aigle, M
rvs突变体表现出与肌动蛋白突变体所描述的表型接近的表型：肌动蛋白细胞骨架的解体、二倍体的随机出芽、极性的丧失和对盐的敏感性。 RVS 基因的突变导致肌动蛋白基因 ACT1 的一系列突变导致合成致死。这种合成致死性对于 act1 突变来说是等位基因特异性的，指向肌动蛋白分子上与肌球蛋白头接触的区域。双突变体 rvs167、myo1 和 rvs167、myo2 分别是致命的并严重影响生长，这一事实进一步加强了肌球蛋白可能参与由 Rvsp 蛋白和肌动蛋白实现的重要功能。这些数据支持这样的观点：肌动蛋白、肌球蛋白和 Rvsp 蛋白在酵母中通过一个共同的功能途径相连。
The rvs mutants display phenotypes close to those described for the actin mutants: disorganization of the actin cytoskeleton, random budding of the diploids, loss of polarity and sensitivity to salt. Mutations in the RVS genes lead to synthetic lethality with a set of mutations in the actin gene, ACT1. This synthetic lethality is allele-specific regarding the act1 mutations, pointing to a region on the actin molecule where contacts with the myosin head have been described. The possible involvement of a myosin in a vital function fulfilled both by the Rvsp proteins and actin is strengthened further by the fact that the double mutants rvs167, myo1 and rvs167, myo2 are lethal and severely affected in growth respectively. These data support the idea that actin, myosin and Rvsp proteins are linked in a common functional pathway in yeast.