Pepper mild mottle virus, a plant virus associated with specific immune responses, Fever, abdominal pains, and pruritus in humans.
Pepper mild mottle virus, a plant virus associated with specific immune responses, Fever, abdominal pains, and pruritus in humans.
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DOI：
10.1371/journal.pone.0010041
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发表时间：
2010-04-06
期刊：
影响因子：
3.7
通讯作者：
Raoult D
中科院分区：
文献类型：
--
作者：
Colson P;Richet H;Desnues C;Balique F;Moal V;Grob JJ;Berbis P;Lecoq H;Harlé JR;Berland Y;Raoult D
最近，宏基因组学研究已经在健康受试者的粪便中鉴定出活的胡椒轻度斑驳病毒（PMMoV），一种植物病毒。然而，其来源和作为病原体的作用尚未确定。使用实时PCR、测序和电子显微镜检测了21种含有辣椒的商品化食品、来自304名成人的357份粪便样本和来自137名儿童的208份粪便样本的PMMoV。同时进行抗PMMoV IgM抗体检测。一项病例对照研究测试了生物学和临床症状与粪便中PMMoV存在的相关性。12种（57%）食品对PMMoV RNA测序呈阳性。粪便样本从22（7.2%）成人和1名儿童（0.7%）是阳性PMMoV的实时PCR。阳性病例更有可能是在皮肤科病房采样的（p<10−6），抗PMMoV IgM抗体血清阳性（p = 0.026），以及表现出发热、腹痛和瘙痒的患者（p分别为0.045、0.038和0.046）。 我们的研究确定了PMMoV的本地来源，并将粪便中PMMoV RNA的存在与特异性免疫反应和临床症状联系起来。虽然临床症状可能归因于另一种辅因子，包括辛辣食物，但我们的数据表明植物病毒在人类中可能具有直接或间接的致病作用。
Recently, metagenomic studies have identified viable Pepper mild mottle virus (PMMoV), a plant virus, in the stool of healthy subjects. However, its source and role as pathogen have not been determined. 21 commercialized food products containing peppers, 357 stool samples from 304 adults and 208 stool samples from 137 children were tested for PMMoV using real-time PCR, sequencing, and electron microscopy. Anti-PMMoV IgM antibody testing was concurrently performed. A case-control study tested the association of biological and clinical symptoms with the presence of PMMoV in the stool. Twelve (57%) food products were positive for PMMoV RNA sequencing. Stool samples from twenty-two (7.2%) adults and one child (0.7%) were positive for PMMoV by real-time PCR. Positive cases were significantly more likely to have been sampled in Dermatology Units (p<10−6), to be seropositive for anti-PMMoV IgM antibodies (p = 0.026) and to be patients who exhibited fever, abdominal pains, and pruritus (p = 0.045, 0.038 and 0.046, respectively). Our study identified a local source of PMMoV and linked the presence of PMMoV RNA in stool with a specific immune response and clinical symptoms. Although clinical symptoms may be imputable to another cofactor, including spicy food, our data suggest the possibility of a direct or indirect pathogenic role of plant viruses in humans.
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影响因子：
3.1
作者：
Near, KA;Stowers, AW;Kaslow, DC
通讯作者：
Kaslow, DC
DOI：
10.1016/j.micron.2005.10.001
发表时间：
2006
期刊：
Micron (Oxford, England : 1993)
影响因子：
--
作者：
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通讯作者：
Dowsett B
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作者：
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Tarr, Phillip I.
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影响因子：
6.7
作者：
Finkbeiner, Stacy R.;Allred, Adam F.;Tarr, Phillip I.;Klein, Eileen J.;Kirkwood, Carl D.;Wang, David
通讯作者：
Wang, David
影响因子：
5.4
作者：
Lacasse, Patrick;Denis, Jerome;Lamarre, Alain
通讯作者：
Lamarre, Alain