Dispersion effects in preparative polymethacrylate monoliths operated in radial-flow columns
Dispersion effects in preparative polymethacrylate monoliths operated in radial-flow columns
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DOI：
10.1016/j.jbbm.2006.09.005
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发表时间：
2007-02-23
影响因子：
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通讯作者：
Jungbauer, Alois
中科院分区：
文献类型：
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作者：
Hahn, Rainer;Tscheliessnig, Anne;Jungbauer, Alois
整体介质已被广泛用作快速分析分离小分子、蛋白质、pDNA和病毒的优秀工具。具有大通道的聚甲基丙烯酸酯整料对于捕获大分子如免疫球蛋白、DNA和病毒是有吸引力的。出于制备目的，这些整料以径向流动模式操作。在整料的频带扩展是非常低的，主要是由额外的列效应的贡献。这里使用的模型有一个单一的轴向分散系数，它将额外的柱效应和整体材料的固有谱带扩展合并在一起，以表征蛋白质和pDNA在聚甲基丙烯酸酯离子交换整体材料上的吸附。由于整料的性能不受所应用范围内的速度的影响，并且由于高度有利的吸附等温线，恒定模式模型可以应用于预测假设轴向流用于建模的径向流单元上的制备运行。(c)2006 Elsevier B.V.保留所有权利。
Monolithic media have found widespread use as excellent tools for fast analytical separations of small molecules, proteins, pDNA and viruses. Polymethacrylate monoliths with large channels are attractive for capturing large molecules, like immunoglobulins, DNA, and viruses. For preparative purposes, these monoliths are operated in radial flow mode. Band spreading in monoliths is extremely low and mostly dominated by the contribution of extra column effects. The model used here had a single axial dispersion coefficient which lumps together extra column effects and the intrinsic band spreading of the monolithic material to characterize the adsorption of proteins and pDNA on polymethacrylate ion-exchange monoliths. Due to the fact that the performance of the monolith was unaffected by the velocity within the applied range, and due to highly favourable adsorption isotherms, a constant pattern model could be applied to predict preparative runs on radial flow units assuming axial flow for modelling. (c) 2006 Elsevier B.V. All rights reserved.