Fibroblasts derived from human chronic diabetic wounds have a decreased proliferation rate, which is recovered by the addition of heparin
Fibroblasts derived from human chronic diabetic wounds have a decreased proliferation rate, which is recovered by the addition of heparin
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DOI：
10.1016/s0923-1811(97)00042-x
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发表时间：
1998-01-01
影响因子：
4.6
通讯作者：
Brismar, K
中科院分区：
文献类型：
--
作者：
Hehenberger, K;Kratz, G;Brismar, K
我们研究了非糖尿病和糖尿病（IDDM）患者未损伤皮肤和慢性伤口中成纤维细胞的生长动力学。在细胞饥饿后的第一个24小时期间的DNA测量显示，来自慢性伤口的成纤维细胞，无论是非糖尿病的还是糖尿病的，都显示出降低的粘附和增殖。当在另外48、72和96小时后确定增殖速率时，与来自未受伤皮肤的成纤维细胞相比，在慢性伤口成纤维细胞中发现增殖速率显著降低。此外，我们还研究了肝素、透明质酸和其他肝素样物质对非糖尿病和糖尿病成纤维细胞增殖的影响。我们发现，这些物质刺激人成纤维细胞的增殖，从正常皮肤和慢性伤口测量的DNA含量。用肝素刺激使糖尿病慢性伤口成纤维细胞的增殖正常化。这种作用与血清的存在无关。肝素的作用是剂量依赖性的，在刺激的前24小时最明显。这些结果表明，肝素可能是重要的治疗慢性糖尿病伤口。(C)1998爱思唯尔科学爱尔兰有限公司
We have studied the growth kinetics of fibroblasts derived from uninjured skin and chronic wounds in non-diabetic and diabetic (IDDM) patients. DNA measurements during the first 24 h after cell starvation showed that fibroblasts derived from chronic wounds, both non-diabetic and diabetic, display a decreased adhesion and proliferation. When determining the rate of proliferation after another 48, 72 and 96 h, a significant decrease in the proliferation rate was found in the chronic wound fibroblasts compared to those from uninjured skin. Furthermore, we have investigated the effects of heparin, hyaluronic acid and other heparin-like substances on the proliferation of non-diabetic and diabetic fibroblasts. We found that these substances stimulated the proliferation of human fibroblasts derived from both normal skin and chronic wounds measured as DNA content. Stimulation with heparin normalized the proliferation of the diabetic chronic wound fibroblasts. This effect was independent of the presence of serum. The effect of heparin was dose-dependent and most pronounced during the first 24 h of stimulation. These results suggest that heparin may be of importance in the treatment of chronic diabetic wounds. (C) 1998 Elsevier Science Ireland Ltd.