First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli
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DOI：
10.1007/s004250100557
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发表时间：
2001-07-01
期刊：
影响因子：
4.3
通讯作者：
Zimmer, W
中科院分区：
文献类型：
--
作者：
Miller, B;Oschinski, C;Zimmer, W
首次从白杨中分离到了异戊二烯合成酶的完整功能基因。该基因与已知的柠檬烯和其他单萜烯脱氢酶非常相似，但发现其特异性催化由前体二甲基烯丙基二磷酸形成异戊二烯，与柠檬烯脱氢酶相比，其对于由香叶基二磷酸形成单萜柠檬烯的活性仅为边缘活性。省略编码转运到叶绿体中所必需的信号肽的基因的一部分，导致重组蛋白的异戊二烯形成速率提高。
For the first time, the complete functional gene for isoprene synthase has been isolated from poplar (Populus alba x Populus tremula). The gene was quite similar to known limonene and other monoterpene synthases, but was found to specifically catalyze the formation of isoprene from the precursor dimethylallyl diphosphate with only a marginal activity for the formation of the monoterpene limonene from geranyl diphosphate as compared with limonene synthases. Omitting the part of the gene that putatively encoded the signal peptide necessary for transport into the chloroplast led to an enhanced rate of isoprene formation by the recombinant protein.