The Structural Integrity of Plasmid-Encoded Pgp3 Is Essential for Induction of Hydrosalpinx by Chlamydia muridarum
The Structural Integrity of Plasmid-Encoded Pgp3 Is Essential for Induction of Hydrosalpinx by Chlamydia muridarum
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质粒编码的 Pgp3 的结构完整性对于鼠衣原体诱导输卵管积水至关重要
DOI：
10.3389/fcimb.2019.00013
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发表时间：
2019-02-05
影响因子：
5.7
通讯作者：
Liu, Yuanjun
中科院分区：
文献类型：
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作者：
Huang, Yumeng;Sun, Yina;Liu, Yuanjun
PGP3由三螺旋螺旋线中间结构域连接的球状N和C末端结构域组成。我们先前证明了pgp3是由衣原体Muridarum诱导Hyalosalpinx所必需的。我们构建了含有PGP3 N末端（PGP3 Delta N），C-terminus（PGP3 Delta C），Ormiddle域（PGP3 Delta M）的缩写孢子转化子。 C3H/HEJ和CBA/JMICE感染了PGP3 Delta N或PGP3 Delta M未能在Oviduct组织中诱导Hyalosalpinx。然而，PGP3三角洲C转化诱导的温和氢蛋白pinx在20％的C3H/HEJ小鼠中（严重程度得分为0.2 +/- 0.6）和40％的CBA/JMICE（严重程度得分为0.8 +/- 1.3）。携带PGP3结构域缺失的转化子的致病性减弱与体外生长受损相关，并且在小鼠下生殖道中的感染率显着降低。此外，感染了PGP3域缺陷型转化剂的C3H/HEJ和CBA/J小鼠的卵形组织显示出较少的炎性细胞浸润。因此，质粒编码的PGP3的结构完整性对于Muridarum C.诱导Hyalosalpinx至关重要。
Pgp3 consists of globular N- and C-terminal domains connected by a triple-helical coiled-coil middle domain. We demonstrated previously that Pgp3 is required for induction of hydrosalpinx by Chlamydia muridarum. We constructed C. muridarum transformants harboring deletion of the Pgp3 N-terminus (pgp3Δn), C-terminus (pgp3Δc), or middle domain (pgp3Δm). C3H/HeJ and CBA/J mice infected with pgp3Δn or pgp3Δm failed to induce hydrosalpinx in oviduct tissue. However, the pgp3Δc transformant induced mild hydrosalpinx in 20% of C3H/HeJ mice (severity score 0.2 ± 0.6) and in 40% of CBA/J mice (severity score 0.8 ± 1.3). The attenuated pathogenicity of the transformants harboring Pgp3 domain deletions was correlated with impaired in vitro growth and significantly reduced infectivity in the mouse lower genital tract. Moreover, the oviduct tissue of C3H/HeJ and CBA/J mice infected with the Pgp3-domain-deficient transformants displayed less inflammatory cell infiltration. Thus, the structural integrity of plasmid-encoded Pgp3 is essential for induction of hydrosalpinx by C. muridarum.