Interaction of enteropathogenic and Shiga toxin-producing Escherichia coli and porcine intestinal mucosa:: Role of intimin and Tir in adherence
Interaction of enteropathogenic and Shiga toxin-producing Escherichia coli and porcine intestinal mucosa:: Role of intimin and Tir in adherence
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DOI：
10.1128/iai.73.9.6005-6016.2005
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发表时间：
2005-09-01
影响因子：
3.1
通讯作者：
Fairbrother, JM
中科院分区：
文献类型：
--
作者：
Girard, F;Batisson, I;Fairbrother, JM
使用源自初乳剥夺的新生仔猪的组织的回肠体外器官培养（IVOC）模型已被证明是离体研究猪肠致病性大肠杆菌（EPEC）附着和消失（A/E）表型的有效方法。本研究的目的是调查 intimin 亚型和 Tir 在从不同动物物种中分离出的 EPEC 和产志贺毒素大肠杆菌 (STEC) 粘附到猪肠道 IVOC 中的作用。此外，使用 intimin 和 Tir 缺陷衍生物研究了 intimin 在人 EPEC 菌株 E2348/69 的 Tir 独立粘附中的作用。我们的结果表明，来自不同动物物种和人类的 A/E 大肠杆菌菌株 (AEEC) 在猪回肠 IVOC 中诱导 A/E 表型，并且 intimin 亚型影响 AEEC 菌株的肠道粘附和向性。我们还表明，EPEC 菌株 E2348/69 的 tir 突变体表现出与猪回肠 IVOC 节段的上皮细胞紧密粘附，微绒毛消失，但没有肌动蛋白聚合或基座形成的证据，并且 intimin 似乎参与了这种表型。总的来说，这项研究为猪肠道中存在一种或多种宿主细胞编码的 intimin 受体提供了进一步的证据。
The ileal in vitro organ culture (IVOC) model using tissues originating from colostrum-deprived newborn piglets has proven to be an effective way to study the attaching and effacing (A/E) phenotype of porcine enteropathogenic Escherichia coli (EPEC) ex vivo. The aim of this study was to investigate the role of intimin subtype and Tir in the adherence of EPEC and Shiga-toxin-producing E. coli (STEC), isolated from different animal species, to porcine intestinal IVOC. Moreover, the role of intimin in Tir-independent adherence of the human EPEC strain E2348/69 was investigated using intimin and Tir-deficient derivatives. Our results demonstrated that A/E E. coli strains (AEEC) from various animal species and humans induce the A/E phenotype in porcine ileal IVOC and that intimin subtype influences intestinal adherence and tropism of AEEC strains. We also showed that a tir mutant of EPEC strain E2348/69 demonstrates close adherence to the epithelial cells of porcine ileal IVOC segments, with microvillous effacement but with no evidence of actin polymerization or pedestal formation, and that intimin seems to be involved in this phenotype. Overall, this study provides further evidence for the existence of one or more host-cell-encoded intimin receptor(s) in the pig gut.