Production of rubusoside derivatives by transgalactosylation of various β-galactosidases
Production of rubusoside derivatives by transgalactosylation of various β-galactosidases
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DOI：
10.1271/bbb1961.53.2923
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发表时间：
1989-11
期刊：
影响因子：
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通讯作者：
S. Kitahata;H. Ishikawa;T. Miyata;O. Tanaka
中科院分区：
文献类型：
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作者：
S. Kitahata;H. Ishikawa;T. Miyata;O. Tanaka
通过不同的β-半乳糖苷酶的转半乳糖基化反应分离得到甜茶苷衍生物，并分析了它们的结构。大肠杆菌β-半乳糖苷酶主要产生13-O-β-d-葡萄糖基-19-O-[β-d-半乳糖基-（1→6）-β-d-葡萄糖基]-甜菊醇（RGal-2）。环状芽孢杆菌β-半乳糖苷酶在反应初期主要产生13-O-β-d-葡萄糖基-19-O-[β-d-半乳糖基-（1→4）-β-d-葡萄糖基]-甜菊醇（RGal-1a），然后产生13-O-[β-d-半乳糖基-（1→4）-β-d-葡萄糖基]-19-O-β-d-葡萄糖基-甜菊醇（RGal-1b）。随着这些产物（RGal-1a和RGal-1b）的量的减少，产生RGal-2。
Rubusoside derivatives by transgalactosylation of various β-galactosidases were isolated and their structures were analyzed. Escherichia coli β-galactosidase produced mainly 13-O-β-d-glucosyl-19-O-[β-d-galactosyl-(1→6)-β-d-glucosyl]-steviol (RGal-2). Bacillus circulans β-galactosidase produced mainly 13-O-β-d-glucosyl-19-O-[β-d-galactosyl-(1→4)-β-d-glucosyl]-steviol (RGal-1a) in the early stage of the reaction and then produced 13-O-[β-d-galactosyl-(1→4)-β-d-glucosyl]-19-O-β-d-glucosyl-steviol (RGal-1b). With decreasing the amount of these products (RGal-1a and RGal-1b), RGal-2 was produced.