Development of SNP-based dCAPS markers linked to major head smut resistance quantitative trait locus qHS2.09 in maize
Development of SNP-based dCAPS markers linked to major head smut resistance quantitative trait locus qHS2.09 in maize
复制标题
开发与玉米主要丝黑穗病抗性数量性状基因座 qHS2.09 相关的基于 SNP 的 dCAPS 标记
DOI：
10.1007/s10681-014-1219-9
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发表时间：
2015-03-01
期刊：
影响因子：
1.9
通讯作者：
Wang, Zhenhua
中科院分区：
文献类型：
--
作者：
Di, Hong;Liu, Xianjun;Wang, Zhenhua
丝黑穗病是中国东北春玉米产区的一种严重危害玉米产量和制种损失的病害。在以往的研究中，我们通过联合连锁和关联作图在≈1.00 Mb的区间内定位了位于SNP PZE-102187486和PZE-102188421之间的主效QTLqHS2.09。在本研究中，我们基于已发现的与主要QTL qHS2.09紧密连锁的单核苷酸多态(SNPs)，开发了六个衍生的裂解扩增多态序列(DCAPS)标记。对于同一碱基突变，SNPs和dCAPS产生的等位基因模式一致。用PAGE凝胶电泳法可以检测到抗丝黑穗病的dCAPS标记，可用于玉米自交系Mo17、齐319及其衍生品系的鉴定。在56个玉米自交系的精细定位中对新标记进行了验证，并对其效率进行了评价。这些dCAPS标记可作为玉米育种的分子标记辅助选择。
Head smut is a disease caused severely yield losses both in commercial maize and seed production in the spring maize growing region in northeastern China. In previous study, we located the major QTL qHS2.09 between SNP PZE-102187486 and PZE-102188421 by combined linkage and association mapping within an interval of ≈1.00 Mb. In this study, we report the development of six derived cleaved amplified polymorphic sequence (dCAPS) markers based on discovered single nucleotide polymorphisms (SNPs) closely linked to the major QTL qHS2.09. There was consistent allele pattern produced by SNPs and dCAPS for the same base mutation. The dCAPS marker for head smut resistance trait could be assayed by PAGE gel electrophoresis and be efficient in identification of maize inbred line Mo17, Qi319 and their derivatives. New markers were validated in fine mapping and evaluated the efficiency in 56 maize inbred lines. These dCAPS markers will facilitate as marker-assisted selection for maize breeding program.