Oncogenic miRNAs Identified in Tear Exosomes From Metastatic Breast Cancer Patients
Oncogenic miRNAs Identified in Tear Exosomes From Metastatic Breast Cancer Patients
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DOI：
10.21873/anticanres.14290
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发表时间：
2020-06-01
影响因子：
2
通讯作者：
Sasaki, Ryohei
中科院分区：
文献类型：
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作者：
Inubushi, Sachiko;Kawaguchi, Hiroki;Sasaki, Ryohei
背景/目的：外泌体由正常细胞和癌细胞产生。外泌体存在于癌症患者的血清中，已用于诊断和预后。最近发现非癌症患者的眼泪含有外泌体。在本报告中，我们描述了晚期乳腺癌患者的眼泪。材料和方法：我们在从 5 名转移性乳腺癌患者的泪液中分离的外泌体中发现了致癌 miRNA，并将其与 8 名健康志愿者的泪液外泌体进行了比较。结果：泪液外泌体的外泌体标志物数量显着高于血清外泌体（CD9、CD63）。对泪液外泌体进行定量逆转录聚合酶反应 (qRT-PCR) 和蛋白质印迹分析，以阐明 miRNA 的状态，此前曾报道转移性乳腺癌患者血清中的 miRNA 状态。 qRT-PCR 和蛋白质印迹分析显示，与健康志愿者的泪液外泌体相比，乳腺癌特异性 miR-21 和 miR-200c 在转移性乳腺癌患者的泪液外泌体中高表达。结论：泪液外泌体可以成为转移性乳腺癌以及其他癌症或疾病的诊断和预后生物标志物的潜在来源。
Background/ Aim: Exosomes are produced by normal and cancer cells. Exosomes are found in the serum of cancer patients and have been used for diagnosis and prognosis. Recently tears from non-cancer patients have been found to contain exosomes. In the present report we describe tears from advanced breast-cancer patients. Materials and Methods: We found oncogenic miRNAs in the exosomes isolated from tear fluids obtained from five patients with metastatic breast cancer and compared them with tear exosomes form eight healthy volunteers. Results: Tear exosomes had a significantly higher quantity of exosome markers than serum exosomes (CD9, CD63). Tear exosomes were subjected to quantitative reverse-transcription polymerase reaction (qRT-PCR), and western blot analysis to elucidate the status of miRNAs, previously reported in serum from patients with metastatic breast cancer. qRT-PCR and western-blot analysis revealed that breast-cancer-specific miR-21 and miR-200c were highly expressed in tear exosomes from metastatic breast cancer patients in contrast to tear exosomes from healthy volunteers. Conclusion: Tear exosomes can be a potential source of diagnostic and prognostic biomarkers for metastatic breast cancer, and possibly other cancers or diseases.