Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Interface
Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Interface
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DOI：
10.1007/978-1-4939-1571-2_6
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Gern, James E.
中科院分区：
文献类型：
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作者：
Ashraf, Shamaila;Brockman-Schneider, Rebecca;Gern, James E.
鼻病毒- c （RV-C）是近年来利用分子方法发现的。传统的方法无法检测到它们，因为它们不能在标准细胞培养中生长。目前已经获得了几种RV-C毒株的完整基因组序列，但关于其生物学特性的信息很少。HRV-C最初是在器官培养中培养的，最近，我们开发了一种在人气道气液界面（ALI）分化上皮细胞中培养HRV-C菌株的系统。通过定量RT-PCR确定，这些培养物支持RV-C菌株的高效复制。该系统使研究RV-C菌株的生物学特性成为可能，包括定量研究。
Rhinovirus-C (RV-C) were discovered recently using molecular methods. Classical methods failed to detect them since they could not grow in standard cell culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells of human airway at air-liquid interface (ALI). These cultures supported efficient replication of RV-C strains as determined by quantitative RT-PCR. This system has enabled study of the biological characteristics of RV-C strains, including quantitative research.