Phenotypic and genotypic identification of Candida dubliniensis from subgingival sites in immunocompetent subjects in Argentina
Phenotypic and genotypic identification of Candida dubliniensis from subgingival sites in immunocompetent subjects in Argentina
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DOI：
10.1111/j.1399-302x.2008.00465.x
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发表时间：
2008-12-01
影响因子：
--
通讯作者：
Rosa, A. C.
中科院分区：
文献类型：
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作者：
Jewtuchowicz, V. M.;Mujica, M. T.;Rosa, A. C.
简介： 人们普遍认为，都柏林念珠菌常见于免疫功能低下的患者，例如患有晚期人类免疫缺陷病毒感染的患者的牙周病部位。由于没有阿根廷的可用数据，本研究的目的是通过表型和分子检测的比较，确定居住在阿根廷布宜诺斯艾利斯的免疫功能正常受试者牙周袋中都柏林念珠菌的流行情况。方法：对 180 名免疫功能正常的非吸烟牙周病患者的龈下菌斑样本中回收的酵母进行了研究。通过常规真菌学方法和特异性聚合酶链反应（PCR）测定来鉴定酵母。氟康唑和伏立康唑敏感性研究是根据临床和实验室标准研究所进行的。结果：在分离的 76 种酵母菌中，都柏林念珠菌占 10.5%（n = 8；95% 置信区间 4.7-19.7），相当于所研究患者的 4.4% (8/180)。白色念珠菌是最常分离的酵母菌种。绝大多数都柏林念珠菌分离株对两种抗真菌药物均敏感，只有一个分离株对两种抗真菌药物均耐药。结论：Staib琼脂显微形态学是与PCR最一致的表型方法，可用于选择假定的都柏林念珠菌。这是第一份使用 PCR 鉴定阿根廷免疫功能正常的牙周病患者龈下液中的都柏林念珠菌的报告。根据本文的研究结果，我们确认都柏林念珠菌可以在免疫功能正常的牙周病患者的牙周袋中定植。
Introduction: It is generally recognized that Candida dubliniensis is commonly found in immunocompromised patients, such as those with advanced human immunodeficiency virus infection, at sites of periodontal disease. Since there are no data available for Argentina, the aim of this study was to determine the prevalence of and to identify C. dubliniensis in periodontal pockets from immunocompetent subjects living in Buenos Aires, Argentina, through a comparison of phenotypic and molecular assays.Methods: Yeasts recovered from subgingival plaque samples were studied for 180 immunocompetent non-smoking patients with periodontal disease. Yeasts were identified by conventional mycological methods and by specific polymerase chain reaction (PCR) assay. Fluconazole and voriconazole susceptibility studies were performed in keeping with the Clinical and Laboratory Standards Institute.Results: Among 76 yeasts isolated, C. dubliniensis comprised 10.5% (n = 8; 95% confidence interval 4.7-19.7), which corresponded to 4.4% of patients studied (8/180). C. albicans was the most frequently isolated species of yeast. A great majority of C. dubliniensis isolates was susceptible with only one isolate resistant to both antifungals.Conclusions: Micromorphology on Staib agar was the phenotypic method that was most concordant with PCR and it was useful for selecting presumptive C. dubliniensis. This is the first report to use PCR to identify C. dubliniensis in subgingival fluid from immunocompetent individuals with periodontal disease in Argentina. On the basis of the findings presented here, we confirm that C. dubliniensis can colonize periodontal pockets of immunocompetent patients with periodontal disease.