Isolation of type II pneumocytes by laser flow cytometry.
Isolation of type II pneumocytes by laser flow cytometry.
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通过激光流式细胞术分离 II 型肺细胞。
DOI：
10.1164/arrd.1982.125.3.326
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Shapiro,DL
中科院分区：
文献类型：
--
作者：
Leary,JF;Finkelstein,JN;Notter,RH;Shapiro,DL
本文报道了用多参数激光流式细胞术从成年兔肺中分离纯化的Ⅱ型肺细胞。用弹性蛋白酶和胰蛋白酶从肺中分离细胞，用亲脂性膦-3R染色。然后通过同时测量板层体的高膦-3R荧光染色和低角度光散射的中等强度来分选II型细胞。我们能够以每小时5 × 105个含有板层体的细胞的分选速率获得基本上纯的群体（98%）。细胞分选技术提供了一个有吸引力的替代密度梯度分离的II型细胞，特别是在详细的人口分析。此外，细胞分选方法的改进提供了区分具有很少板层体的分化II型细胞的潜力，所述板层体基于浮力密度不分离。
The isolation of a pure population of type II pneumocytes from adult rabbit lung by the technique of multiparameter laser flow cytometry is reported here. Lipophilic phosphine-3R was used to stain cells dissociated from lungs by elastase and trypsin. Type II cells were then sorted by simultaneous measurement of high phosphine-3R fluorescent staining of lamellar bodies and intermediate intensity of low-angle light scatter. We were able to obtain an essentially pure population (98%) at sorting rates of 5 × 105lamellar-body-containing cells per hour. The cell sorter technique offers an attractive alternative to the density gradient isolation of type II cells, particularly in terms of detailed population analysis. In addition, refinements in cell sorter methods offer the potential to discriminate differentiating type II cells with few lamellar bodies that do not segregate on the basis of buoyant density.