Male and female genotype and a genotype-by-genotype interaction mediate the effects of mating on cellular but not humoral immunity in female decorated crickets
Male and female genotype and a genotype-by-genotype interaction mediate the effects of mating on cellular but not humoral immunity in female decorated crickets
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DOI：
10.1038/s41437-020-00384-8
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发表时间：
2020-11-20
期刊：
影响因子：
3.8
通讯作者：
Sadd, Ben M.
中科院分区：
文献类型：
--
作者：
Hampton, Kylie J.;Duffield, Kristin R.;Sadd, Ben M.
性拮抗协同进化的预测，导致男性和女性基因型的分歧有关的物质转移的影响，男性在交配女性生理。因此，交配的结果应该取决于交配基因型的特定组合。虽然交配已被证明会影响不同昆虫类群中的雌性免疫力，但交配后雄性-雌性基因型对雌性免疫力的影响在很大程度上仍未被探索。在这里，我们调查的影响，交配对女性装饰板球基线免疫和潜在的男性基因型的女性基因型的相互作用，影响这一反应。来自三种不同基因型背景的雌性动物与来自相同三种基因型背景的雄性动物在完全互惠设计中保持未交配或单独交配。对血细胞和血细胞微聚集体进行定量，用于雌性细胞免疫，并对参与黑化的酚氧化酶和体液免疫的抗菌活性进行定量。在该系统中，雌性细胞免疫对交配反应更强，交配效应具有基因型依赖性。具体而言，对于血细胞，交配状态的基因型相互作用介导的交配本身的影响，和一个显着的男性-女性基因型的基因型相互作用确定交配后血细胞耗竭。微聚集的影响，女性的基因型或她的配偶。雌性体液免疫指标未受影响，表明交配后对雌性的影响倾向取决于基线免疫力的组成部分。血细胞的基因型的基因型效应支持交配后免疫抑制的性冲突的作用，这表明分歧的男性基因型相对于修改女性交配后的免疫力，和分歧的女性基因型抵抗这些影响。
Sexually antagonistic coevolution is predicted to lead to the divergence of male and female genotypes related to the effects of substances transferred by males at mating on female physiology. The outcome of mating should thus depend on the specific combination of mating genotypes. Although mating has been shown to influence female immunity in diverse insect taxa, a male-female genotype-by-genotype effect on female immunity post mating remains largely unexplored. Here, we investigate the effects of mating on female decorated cricket baseline immunity and the potential for a male-genotype-by-female-genotype interaction affecting this response. Females from three distinct genotypic backgrounds were left unmated or singly mated in a fully reciprocal design to males from the same three genotypic backgrounds. Hemocytes and hemocyte microaggregations were quantified for female cellular immunity, and phenoloxidase, involved in melanization, and antibacterial activity for humoral immunity. In this system, female cellular immunity was more reactive to mating, and mating effects were genotype-dependent. Specifically, for hemocytes, a genotype-by-mating status interaction mediated the effect of mating per se, and a significant male-female genotype-by-genotype interaction determined hemocyte depletion post mating. Microaggregations were influenced by the female's genotype or that of her mate. Female humoral immune measures were unaffected, indicating that the propensity for post-mating effects on females is dependent on the component of baseline immunity. The genotype-by-genotype effect on hemocytes supports a role of sexual conflict in post-mating immune suppression, suggesting divergence of male genotypes with respect to modification of female post-mating immunity, and divergence of female genotypes in resistance to these effects.