MafA differentiates rat intestinal cells into insulin-producing cells
MafA differentiates rat intestinal cells into insulin-producing cells
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DOI：
10.1016/j.bbrc.2006.08.032
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发表时间：
2006-10-13
影响因子：
3.1
通讯作者：
Kashiwagi, Atsunori
中科院分区：
文献类型：
--
作者：
Nomura, Satoshi;Nakamura, Takaaki;Kashiwagi, Atsunori
最近的研究表明，碱性亮氨酸拉链转录因子MafA在胰腺β细胞特异性胰岛素基因转录中具有关键作用。因此，我们研究了MafA在肠中的过表达是否诱导体内小肠上皮细胞中的胰岛素产生。制备含MafA基因的重组腺病毒（Ad-MafA），并经口给予链脲佐菌素（STZ）治疗的糖尿病大鼠。RT-PCR分析肠道中胰岛素基因的表达，然后免疫组织化学分析Ad-MafA后检测胰岛素蛋白。此外，MafA在肠中的过表达增加血浆胰岛素水平并改善高血糖症。这些结果表明，MafA在肠中的过表达诱导肠上皮细胞新产生和释放胰岛素。(c)2006年爱思唯尔公司All rights reserved.
Recent studies have suggested that basic leucine zipper transcription factor MafA has a crucial role in pancreatic beta-cell-specific insulin gene transcription. Thus, we investigated whether MafA overexpression in the intestine induces insulin production in small-intestinal epithelial cells in vivo. Recombinant adenovirus containing MafA gene (Ad-MafA) was prepared and administered orally to streptozo-cin-treated diabetic rats. Insulin gene expression was observed in the intestine by RT-PCR analysis, and then insulin protein was detected by immunohistochemical analysis after Ad-MafA administration. Furthermore, MafA overexpression in the intestine increased plasma insulin levels and ameliorated hyperglycemia. These results indicate that MafA overexpression in the intestine induces intestinal epithelial cells newly to produce and release insulin. (c) 2006 Elsevier Inc. All rights reserved.