ABO genotyping following a single PCR amplification.
ABO genotyping following a single PCR amplification.
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单次 PCR 扩增后进行 ABO 基因分型。
DOI：
10.1520/jfs15425j
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发表时间：
1996
影响因子：
1.6
通讯作者：
K. Kimura
中科院分区：
文献类型：
--
作者：
A. Akane;S. Yoshimura;M. Yoshida;Y. Okii;T. Watabiki;K. Matsubara;K. Kimura
利用Lee和Chang设计的引物，用PCR扩增出了横跨O等位基因单核苷酸缺失位点的200碱基对（bp） ABO位点片段。PCR产物经Kpn I酶切可鉴定出O等位基因。对产物的Mae II酶切也可区分A和B等位基因。由此可见，单次扩增后，Kpn I和Mae II酶切可分型ABO血型。在O等位基因中也发现了A和B之间200 bp产物的核苷酸替换，形成了OA和OG两个不同的亚等位基因。在单次扩增后，对PCR产物的单链构象多态性进行ABO基因分型研究。
Using primers designed by Lee and Chang, 200 base-pair (bp) fragment of ABO locus was amplified by PCR, which spans the site of the single nucleotide deletion associated with O allele. O allele could be identified by Kpn I digestion of the PCR product as reported. A and B alleles were also distinguishable by Mae II digestion of the product. Thus restriction digestion by Kpn I and Mae II could genotype ABO blood group following the single amplification. The nucleotide substitution in the 200-bp product between A and B alleles was also found in O allele, resulting in 2 different suballeles OA and OG. The single-strand conformational polymorphism of the PCR product was also investigated for ABO genotyping following the single amplification.
DOI：
10.1016/s0021-9258(19)40170-1
发表时间：
1990-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Yamamoto;J. Marken;T. Tsuji;T. White;H. Clausen;S. Hakomori
通讯作者：
F. Yamamoto;J. Marken;T. Tsuji;T. White;H. Clausen;S. Hakomori