Surface plasmon resonance analysis at a supported lipid monolayer
Surface plasmon resonance analysis at a supported lipid monolayer
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DOI：
10.1016/s0005-2736(98)00091-1
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发表时间：
1998-08-14
影响因子：
3.4
通讯作者：
Williams, DH
中科院分区：
文献类型：
--
作者：
Cooper, MA;Try, AC;Williams, DH
描述了在表面等离子体共振仪器中在疏水性自组装单层的顶部上形成支撑的脂质单层的方法。小的单层囊泡自发地吸收到疏水性自组装单层的表面以形成类似于细胞膜表面的表面。亲脂配体，例如小酰化肽或糖基磷脂酰肌醇锚定蛋白，被插入到吸收的脂质中，并且通过表面等离子体共振分析分析物与这些配体的结合。脂质单分子层形成的条件已经在脂质类型、化学和缓冲液相容性、配体稳定性和再现性方面进行了优化。(C)1998 Elsevier Science B.V.保留所有权利。
Methods for the formation of supported lipid monolayers on top of a hydrophobic self assembled monolayer in a surface plasmon resonance instrument are described. Small unilamellar vesicles absorb spontaneously to the surface of the hydrophobic self-assembled monolayer to form a surface which resembles the surface of a cellular membrane, Lipophilic ligands, such as small acylated peptides or glycosylphosphatidylinositol-anchored proteins, were inserted into the absorbed lipid and binding of analytes to these ligands was analysed by surface plasmon resonance. Conditions for the formation of lipid monolayers have been optimised with respect to lipid type, chemical and buffer compatibility, ligand stability and reproducibility. (C) 1998 Elsevier Science B.V. All rights reserved.