Guanylyl cyclase expression in specific sensory neurons: A new family of chemosensory receptors
Guanylyl cyclase expression in specific sensory neurons: A new family of chemosensory receptors
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DOI：
10.1073/pnas.94.7.3384
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发表时间：
1997-04-01
影响因子：
11.1
通讯作者：
Garbers, DL
中科院分区：
文献类型：
--
作者：
Yu, S;Avery, L;Garbers, DL
鸟苷酸环化酶（GC-D）最近被证明在大鼠神经上皮内的神经元亚类中表达，但是考虑到仅发现了单一的环化酶，其是否代表气味剂/信息素受体，如对于七跨膜受体的大家族所建议的，仍然不清楚，通过克隆和表达的cDNA，我们现在证明，至少有29个基因组或cDNA序列中发现的秀丽隐杆线虫鸟苷酸环化酶。许多膜形式保留已知环化酶受体的胞外配体结合结构域内保守的半胱氨酸残基。获得信号的孤儿环化酶受体：：GFP（绿色荧光蛋白）融合构建体的全部在特定感觉神经元中表达。此外，环化酶/GFP融合蛋白（GCY-10/GFP）主要在感觉纤毛中表达，表明这些环化酶作为初级化学感受受体发挥作用。我们还首次发现，化学感觉神经元（ASE），已知他双侧对称，表现出绝对的右侧或左侧相对于三种不同的环化酶的表达。因此，鸟苷酸环化酶代表了一个意想不到的大的和新的家族的感觉神经元受体，可以补充7-跨膜家族的气味/信息素受体。
A guanylyl cyclase (GC-D) was recently shown to be expressed in a subclass of neurons within the neuroepithelim of the rat, but given that only a single cyclase was discovered, whether it represents an odorant/pheromone receptor as has been suggested for the large family of seven-transmembrane receptors remains unclear, Through cloning and expression of cDNA we now demonstrate that at least 29 genomic or cDNA sequences found in Cacnorhabditis elegans represent guanylyl cyclases. Many of the membrane forms retain cysteine residues conserved within the extracellular, ligand-binding domain of known cyclase receptors. Of fight orphan cyclase receptor::GFP (green fluroescence protein) fusion constructs for which signals were obtained, all were expressed in specific sensory neurons. Furthermore, a cyclase/GFP fusion protein (GCY-10/GFP) was principally expressed in the sensory cilium, suggesting these cyclases function as primary chemosensory receptors. For the first time, we also found that chemosensory neurons (ASE), known to he bilaterally symmetric, demonstrate absolute right ol left sidedness with respect to the expression of three different cyclases. Thus, the guanylyl cyclases represent an unexpectedly large and new family of sensory neuron receptors that may complement the 7-transmembrane family of odorant/pheromone receptors.