X-ray dose delivered during a longitudinal micro-CT study has no adverse effect on cardiac and pulmonary tissue in C57BL/6 mice
X-ray dose delivered during a longitudinal micro-CT study has no adverse effect on cardiac and pulmonary tissue in C57BL/6 mice
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DOI：
10.1177/0284185113475608
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发表时间：
2013-05-01
期刊：
影响因子：
1.3
通讯作者：
Drangova, Maria
中科院分区：
文献类型：
--
作者：
Detombe, Sarah A.;Dunmore-Buyze, Joy;Drangova, Maria
背景：微型计算机断层扫描(Micro-CT)为小动物成像提供了许多优势，包括在整个纵向研究中监视相同的动物的能力。目的：对C57BL/6小鼠进行为期6周的每周多次扫描，以确定累积剂量对小鼠肺和心脏组织的影响。材料和方法：将C57BL/6雄性小鼠分为两组(照射组10只，对照组10只)。照射组每周扫描3次(80kVP/50 mA)，共6周，每周剂量为0.84GY，总剂量为5.04GY。对照组在最后一周接受扫描。结果：照射组肺体积、肺密度、左心室容积、射血分数总体上与对照组无显著差异。照射组小鼠肺和心肌组织的组织学标本也未显示炎症或纤维化的迹象。结论：本研究表明，在纵向显微CT研究中，累积超过6周的5GyX射线剂量对C57BL/6小鼠的肺和心肌组织没有显著影响。因此，微型CT成像的许多优势，包括在自由呼吸的小鼠身上快速获取高分辨率、各向同性的图像，可以在纵向研究中得到利用，而不需要考虑与剂量相关的负面影响。
Background: Micro-computed tomography (micro-CT) offers numerous advantages for small animal imaging, including the ability to monitor the same animals throughout a longitudinal study. However, concerns are often raised regarding the effects of X-ray dose accumulated over the course of the experiment.Purpose: To scan C57BL/6 mice multiple times per week for 6 weeks, in order to determine the effect of the cumulative dose on pulmonary and cardiac tissue at the end of the study.Material and Methods: C57BL/6 male mice were split into two groups (irradiated group 10, control group 10). The irradiated group was scanned (80 kVp/50mA) three times weekly for 6 weeks, resulting in a weekly dose of 0.84 Gy, and a total study dose of 5.04 Gy. The control group was scanned on the final week. Scans from week 6 were reconstructed and the lungs and heart were analyzed.Results: Overall, there was no significant difference in lung volume or lung density or in left ventricular volume or ejection fraction between the control group and the irradiated group. Histological samples taken from excised lung and myocardial tissue also showed no evidence of inflammation or fibrosis in the irradiated group.Conclusion: This study demonstrated that a 5 Gy X-ray dose accumulated over 6 weeks during a longitudinal micro-CT study had no significant effects on the pulmonary and myocardial tissue of C57BL/6 mice. As a result, the many advantages of micro-CT imaging, including rapid acquisition of high-resolution, isotropic images in free-breathing mice, can be taken advantage of in longitudinal studies without concern for negative dose-related effects.