CD8(+) T cells mediate CD40-independent maturation of dendritic cells in vivo.
CD8(+) T cells mediate CD40-independent maturation of dendritic cells in vivo.
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DOI：
10.1084/jem.189.12.1875
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发表时间：
1999-06-21
期刊：
影响因子：
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通讯作者：
Bachmann MF
中科院分区：
文献类型：
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作者：
Ruedl C;Kopf M;Bachmann MF
针对次要组织相容性抗原的细胞毒性T淋巴细胞(CTL)反应的诱导依赖于T细胞帮助的存在，并需要树突状细胞(DC)上的CD40与活化的T辅助细胞(Th)上的CD40配体的相互作用。本研究表明CD40既不参与Th依赖的抗病毒CTL反应，也不参与Th非依赖的抗病毒CTL反应。此外，数据表明，在缺乏CD40和Th的情况下，DC在病毒感染后在体内发生成熟。这种成熟不需要 树突状细胞的病毒感染，而是由多肽特异的CD8T细胞介导的。令人惊讶的是，在体内和体外，幼稚的CD8+T细胞能够在激活后24小时内触发DC成熟。此外，多肽激活的CD8+T细胞能够诱导反式成熟，因为在体内未能呈递相关抗原的DC也经历了成熟。在分离后，体内刺激的DC能够在体外将经典的Th依赖的CTL反应(抗HY)转化为Th非依赖的反应。因此，在缺乏CD40的情况下，抗病毒CD8+T细胞足以使DC成熟。
Induction of cytotoxic T lymphocyte (CTL) responses against minor histocompatibility antigens is dependent upon the presence of T cell help and requires the interaction of CD40 on dendritic cells (DCs) with CD40 ligand on activated T helper cells (Th). This study demonstrates that CD40 is neither involved in Th-dependent nor Th-independent antiviral CTL responses. Moreover, the data show that DC maturation occurs in vivo after viral infection in the absence of CD40 and Th. This maturation did not require viral infection of DCs but was mediated by peptide-specific CD8+ T cells. Surprisingly, naive CD8+ T cells were able to trigger DC maturation within 24 h after activation in vivo and in vitro. Moreover, peptide-activated CD8+ T cells were able to induce maturation in trans, as DCs that failed to present the relevant antigen in vivo also underwent maturation. Upon isolation, the in vivo–stimulated DCs were able to convert a classically Th-dependent CTL response (anti-HY) into a Th-independent response in vitro. Thus, antiviral CD8+ T cells are sufficient for the maturation of DCs in the absence of CD40.