Finding nuclear localization signals
Finding nuclear localization signals
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DOI：
10.1093/embo-reports/kvd092
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发表时间：
2000-11-01
期刊：
影响因子：
7.7
通讯作者：
Rost, B
中科院分区：
文献类型：
--
作者：
Cokol, M;Nair, R;Rost, B
各种各样的核定位信号（NLS）的实验是已知的，虽然只有一个基序可用于数据库搜索通过PROSITE。我们最初从文献中收集了一组91个实验验证的NLS。通过反复的“计算机诱变”，我们将这一组扩展到214个潜在的NLS。这最后一组在所有已知的核蛋白中有43%匹配，而在已知的非核蛋白中没有匹配。我们估计，所有真核生物蛋白质的>17%可以被输入到细胞核中。最后，我们发现90%的蛋白质的NLS和DNA结合区域之间的重叠，其中NLS和DNA结合区域是已知的。因此，当DNA结合蛋白进入细胞核时，进化似乎已经使用了现有的DNA结合机制的一部分。然而，在我们的214个NLS基序中，只有56个与DNA结合区重叠。这56个NLS使从头预测的部分DNA结合区域的类似800蛋白质在人类，苍蝇，蠕虫和酵母。
A variety of nuclear localization signals (NLSs) are experimentally known although only one motif was available for database searches through PROSITE. We initially collected a set of 91 experimentally verified NLSs from the literature. Through iterated 'in silico mutagenesis' we then extended the set to 214 potential NLSs. This final set matched in 43% of all known nuclear proteins and in no known non-nuclear protein. We estimated that >17% of all eukaryotic proteins may be imported into the nucleus. Finally, we found an overlap between the NLS and DNA-binding region for 90% of the proteins for which both the NLS and DNA-binding regions were known. Thus, evolution seemed to have used part of the existing DNA-binding mechanism when compartmentalizing DNA-binding proteins into the nucleus. However, only 56 of our 214 NLS motifs overlapped with DNA-binding regions. These 56 NLSs enabled a de novo prediction of partial DNA-binding regions for similar to 800 proteins in human, fly, worm and yeast.