Butyric acid induces apoptosis in inflamed fibroblasts
Butyric acid induces apoptosis in inflamed fibroblasts
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DOI：
10.1177/154405910808700108
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发表时间：
2008-01-01
影响因子：
7.6
通讯作者：
Ochiai, K.
中科院分区：
文献类型：
--
作者：
Kurita-Ochiai, T.;Seto, S.;Ochiai, K.
丁酸，牙周病细菌的细胞外代谢产物，诱导小鼠和人类T-和B-细胞凋亡，而从健康人中分离的完整牙龈成纤维细胞对丁酸诱导的细胞凋亡有抵抗力。我们研究了从成人牙周炎患者中分离的发炎牙龈成纤维细胞对丁酸诱导的细胞凋亡的敏感性。丁酸显着抑制发炎牙龈成纤维细胞的活力，并诱导细胞凋亡的剂量依赖性方式。炎症牙龈成纤维细胞与丁酸孵育诱导DNA断裂和凋亡的变化，如染色质凝聚，亚二倍体细胞核，线粒体损伤。此外，丁酸诱导的发炎牙龈成纤维细胞凋亡减少半胱天冬酶-3/7，-6，-8，和-9抑制剂。因此，与健康牙龈成纤维细胞相比，成年牙周炎患者发炎牙龈成纤维细胞似乎对丁酸诱导的线粒体和半胱天冬酶依赖性细胞凋亡高度敏感。
Butyric acid, an extracellular metabolite from periodontopathic bacteria, induces apoptosis in murine and human T- and B-cells, whereas intact gingival fibroblasts isolated from healthy humans are resistant to butyric-acid-induced apoptosis. We examined the susceptibility of inflamed gingival fibroblasts isolated from adult persons with periodontitis to butyric-acid-induced apoptosis. Butyric acid significantly suppressed the viability of inflamed gingival fibroblasts and induced apoptosis in a dose-dependent manner. The incubation of inflamed gingival fibroblasts with butyric acid induced DNA fragmentation and apoptotic changes such as chromatin condensation, hypodiploid nuclei, and mitochondrial injury. Furthermore, butyric-acid-induced apoptosis in inflamed gingival fibroblasts was reduced by caspase-3/7, -6, -8, and -9 inhibitors. Thus, inflamed gingival fibroblasts from adult persons with periodontitis appear to be highly susceptible to mitochondria- and caspase-dependent apoptosis induced by butyric acid, compared with healthy gingival fibroblasts.