Local cytokine response in Helicobacter pylori-infected subjects
Local cytokine response in Helicobacter pylori-infected subjects
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DOI：
10.1128/iai.66.12.5964-5971.1998
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发表时间：
1998-12-01
影响因子：
3.1
通讯作者：
Svennerholm, AM
中科院分区：
文献类型：
--
作者：
Lindholm, C;Quiding-Järbrink, M;Svennerholm, AM
宿主对幽门螺杆菌感染的免疫应答可能对该生物体感染的结果具有重要意义，例如，来解释为什么只有一部分受感染的人会患上消化性溃疡。在这项研究中，我们分析了不同细胞因子的局部反应，即，促炎性白细胞介素-1 β（IL-1 β）、IL-6、肿瘤坏死因子α和IL-8;免疫调节γ干扰素（IFN-γ）和IL-4;以及抗炎性转化生长因子β（TGF-β）--在来自H.幽门感染的十二指肠鹿（DU）患者和无症状（AS）携带者（即，慢性胃炎）。为了比较，还分析了来自未感染的健康个体的活检标本。免疫组织化学技术用于定量细胞因子应答以及鉴定与细胞因子表达相关的细胞类型。我们发现，除IL-4外，所有研究的细胞因子水平在H。幽门螺杆菌感染的受试者与健康个体的水平相比。我们的结果表明，窦细胞因子反应是Th型的，因为IFN-γ，而不是IL-4，在H.幽门螺杆菌感染的DU患者和AS携带者。然而，当H.比较了有和没有消化性溃疡的幽门感染受试者。一些细胞因子，特别是IL-1 β和TGF-β，也发现在胃粘膜的健康，未感染的受试者。我们还发现，胃上皮细胞对促炎细胞因子IL-1 β和IL-6以及IL-8的胃窦细胞因子应答有很大贡献。
The host immune response to Helicobacter pylori infection might be of importance with regard to the outcome of infection by this organism, e.g., to explain why only a proportion of infected subjects develop peptic ulcers. In this study we have analyzed the local response of different cytokines-i.e., the proinflammatory interleukin-1 beta, (IL-1 beta), IL-6, tumor necrosis factor alpha, and IL-8; the immunoregulatory gamma interferon (IFN-gamma); and IL-4; and the anti-inflammatory transforming growth factor beta (TGF-beta)-in antral biopsy specimens from H. pylori-infected duodenal deer (DU) patients and asymptomatic (AS) carriers (i.e., with chronic gastritis only). For comparison, biopsy specimens from uninfected healthy individuals were also analyzed. An immunohistochemical technique was used to allow quantification of the cytokine responses as well as identification of the cell types associated with the cytokine expression. We found that the levels of all of the studied cytokines except IL-4 were increased in the H. pylori-infected subjects compared to the levels in the healthy individuals. Our results indicate that the antral cytokine response is of the Th, type since IFN-gamma, but not IL-4, was up-regulated bath in H. pylori-infected DU patients and in AS carriers. However, there were no significant differences in either proinflammatory or immunoregulatory cytokine levels when H. pylori-infected subjects with and without peptic ulcers were compared. Some of the cytokines, particularly IL-1 beta and TGF-beta, were also found in the gastric mucosae of healthy, uninfected subjects. We also showed that the gastric epithelium contributes substantially to the antral cytokine response of the proinflammatory cytokines IL-1 beta and IL-6 in addition to IL-8.