EFFECT OF ACUTE ETHANOL INVIVO AND INVITRO ON THE BETA-ENDORPHIN SYSTEM IN THE RAT
EFFECT OF ACUTE ETHANOL INVIVO AND INVITRO ON THE BETA-ENDORPHIN SYSTEM IN THE RAT
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DOI：
10.1016/0024-3205(87)90247-5
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发表时间：
1987-01-05
期刊：
影响因子：
6.1
通讯作者：
BARCOMB, A
中科院分区：
文献类型：
--
作者：
GIANOULAKIS, C;BARCOMB, A
体内急性乙醇处理（i.p注射3.5 g乙醇/Kg B.重量）刺激β的释放，脑下垂体释放内啡肽样肽，如β-内啡肽含量增加所示。内啡肽样免疫反应性（β- EPLIR）在血浆中。此外，β-肾上腺素前叶含量的显著降低观察到EPLIR，而神经中间叶β-EPLIR的降低则与神经中间叶β-EPLIR的降低有关。腹膜内乙醇注射后45分钟的EPLIR含量无统计学显著性。在3 H苯丙氨酸存在下，来自注射乙醇或盐水的动物的神经中间叶的体外孵育表明β-苯丙氨酸的含量增加。孵育培养基中的EPLIR增加，神经中间叶提取物中新生物合成的3 H-苯丙氨酸标记的蛋白质的含量降低，而3 H-苯丙氨酸标记的阿黑皮素原、β-阿黑皮素原和β-阿黑皮素原的含量降低。促脂素和β-神经中间叶提取物中的内啡肽通过乙醇处理没有显著改变，尽管观察到少量增加。当来自未处理的对照动物的神经中间叶与3 H-苯丙氨酸在每100 ml孵育培养基中存在或不存在300 mg乙醇的情况下孵育3小时时，β-谷氨酰胺的浓度没有显著差异。孵育培养基中的EPLIR含量，或3 H-苯丙氨酸标记的蛋白质、阿黑皮素原、β-促脂素和β-内啡肽在神经中间叶提取物。这些结果表明，乙醇对β-葡萄糖苷酶几乎没有或没有直接影响。中间部细胞内的内啡肽。
Acute ethanol treatment in vivo (i.p injection of 3.5 g ethanol/Kg B. wt.) stimulated the release of .beta.-endorphin like peptides by the pituitary gland as was indicated by the increased content of .beta.-endorphin like immunoreactivity (.beta.-EPLIR) in the plasma. Furthermore, a significant decrease in the anterior lobe content of .beta.-EPLIR was observed, while the decrease in the neurointermediate lobe .beta.-EPLIR content at 45 min after the i.p. ethanol injection was not statistically significant. In vitro incubation of neurointermediate lobes, from animals injected with either ethanol or saline, in the presence of 3H phenylalanine indicated that the content of .beta.-EPLIR in the incubation medium was increased, the content of the newly biosynthesized 3H-phenylalanine labelled proteins in the neurointermediate lobe extract was decreased, while the content of 3H-phenylalanine labelled pro-opiomelanocortin, .beta.-lipotropin and .beta.-endorphin in the neurointermediate lobe extract were not significantly changed by the ethanol treatment, though a small increase was observed. When neurointermediate lobes from untreated control animals were incubated for 3 hrs with 3H-phenylalanine in the presence or absence of 300 mg ethanol per 100 ml incubation medium, there was no signifiant difference in the .beta.-EPLIR content in the incubation medium, or in the content of 3H-phenylalanine labelled proteins, pro-opiomelanocortin, .beta.-lipotropin and .beta.-endorphin in the neurointermediate lobe extract. These results suggest that ethanol has little or no direct effect on the .beta.-endorphin peptides in the pars intermedia cells.