Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins
Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins
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DOI：
10.1093/jxb/erg160
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发表时间：
2003-06-01
影响因子：
6.9
通讯作者：
Di Sansebastiano, GP
中科院分区：
文献类型：
--
作者：
Flückiger, R;De Caroli, M;Di Sansebastiano, GP
绿色荧光蛋白（GFP）允许基因表达的直接可视化和融合蛋白在活细胞中的亚细胞定位。在稳定转化的拟南芥中，研究了不同GFP融合蛋白在分泌系统中的定位。Wassilewskaja。分泌型GFP （SGFP）和内质网中保留的GFP （GFP- kdel）证实了已知的模式，但两个液泡型GFP （GFP- chi和Aleu-GFP）首次标记了拟南芥液泡系统，其组织似乎依赖于细胞分化。液泡中绿色荧光蛋白的稳定性可能取决于pH值或降解程度，但这些液泡标记物可以作为生理研究的工具，使这些植物适合于诱变和基因标记实验。
Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two vacuolar GFPs (GFP-Chi and Aleu-GFP) labelled the Arabidopsis vacuolar system for the first time, the organization of which appears to depend on cell differentiation. GFP stability in the vacuoles may depend on pH or degradation, but these vacuolar markers can, nevertheless, be used as a tool for physiological studies making these plants suitable for mutagenesis and gene-tagging experiments.