Tumor suppressor miR-149-5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma
Tumor suppressor miR-149-5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma
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DOI：
10.3892/mmr.2016.5205
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发表时间：
2016-06-01
影响因子：
3.4
通讯作者：
Lai, Yongqing
中科院分区：
文献类型：
--
作者：
Jin, Lu;Li, Yifan;Lai, Yongqing
Several studies have recently explored the role of microRNAs (miRNAs, miRs) in the tumorigenesis of various types of cancer. miRNAs have been reported to be involved in numerous cell processes, including cell apoptosis, proliferation and migration, thus suggesting that miRNAs may have an important role in cancer progression. Downregulation of miR-149-5p has been detected in RCC tissues by microarray profiling; however, its expression and function in RCC has yet to be elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction was performed to detect the expression levels of miR-149-5p in RCC tissues and paired normal tissues. In order to determine whether miR-149-5p was able to regulate cell proliferation, apoptosis or migration, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide, flow cytometric and wound healing assays were conducted. The results demonstrated that miR-149-5p was significantly downregulated in RCC tissues compared with in normal tissues (P
