Molecular cloning and expression of rat contraception associated protein 1 (CAP1), a protein putatively involved in fertilization.
Molecular cloning and expression of rat contraception associated protein 1 (CAP1), a protein putatively involved in fertilization.
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大鼠避孕相关蛋白 1 (CAP1) 的分子克隆和表达，该蛋白被认为与受精有关。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Trevor G. Cooper
中科院分区：
文献类型：
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作者：
A. Wagenfeld;Jörg Gromoll;Trevor G. Cooper
口服奥硝唑导致不育的大鼠附睾液中含有一种CAP1(避孕药相关蛋白1)蛋白，这种蛋白在附睾液中不存在，但存在于服用可育药物的大鼠的附睾液中。从大鼠睾丸cDNA文库中克隆了cap1基因，并对其在不同组织中的表达进行了研究。推导的蛋白序列包含189个氨基酸残基，数据库搜索显示与人和小鼠DJ-1在核苷酸和氨基酸水平上有很高的同源性(83-95%)。从大鼠不同组织的Northern印迹杂交显示，Cap1由1.6kb的RNA转录本编码，似乎在大鼠体内普遍表达，并在睾丸中高水平表达。
Epididymal fluid from rats rendered infertile by oral administration of ornidazole contains a protein CAP1 (contraception-associated protein 1) that is absent from epididymal fluid, but present on epididymal sperm, from fertile vehicle-treated rats. The gene for CAP1 has been isolated from a rat testis cDNA library and its expression investigated in different tissues. The deduced protein sequence of CAP1 contains 189 amino-acid residues and database searches revealed a high degree of homology (83-95%) with human and mouse DJ-1 at the nucleotide and amino acid levels. Northern blot hybridisation from different rat tissues indicated that CAP1 is encoded by a 1.6-kilobase RNA transcript and seems to be ubiquitiously expressed in the rat with a high level of expression in the testis.