Effect of sample matrices on supported liquid membrane: Efficient electromembrane extraction of cathinones from biological samples
Effect of sample matrices on supported liquid membrane: Efficient electromembrane extraction of cathinones from biological samples
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样品基质对支撑液膜的影响：从生物样品中高效电膜萃取卡西酮
DOI：
10.1016/j.talanta.2021.123175
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Chuixiu Huang
中科院分区：
文献类型：
--
作者：
Changbao Hong;Ying Dong;Ruiqin Zhu;Yibo Yan;Xiantao Shen;Stig Pedersen-Bjergaard;Chuixiu Huang
本文首次以卡西酮（log P < 1.0）为极性基本模型物，研究了样品基质对电膜萃取的影响。分别检测了10种支撑液膜（SLM）的加标缓冲液、尿液和全血样品的EME。对于缓冲溶液，含有芳香族溶剂的SLM提供了比非芳香族溶剂更高的EME回收率，这证实了阳离子-π相互作用对碱性物质的EME的重要性。有趣的是，当应用于尿液和全血样品时，芳香族SLM的效率较低，而含有丰富氢键酸碱性的非芳香族SLM是有效的。这些观察结果被解释为SLM结垢，并且SLM的疏水性明显取决于SLM溶剂的性质。因此，开发了含有芳香族1-乙基-2-硝基苯（ENB）和非芳香族1-十一烷醇（1：1 v/v）的二元SLM。该二元SLM不易于结垢，并且提供了从尿液和全血中高的卡西酮回收率。将该方法与液相色谱-串联质谱（LC-MS/MS）联用，对全血和尿液中卡西酮的线性范围分别为5-200 ng/mL和1-200 ng/mL，R2≥ 0.9903。卡西酮的LOD和LOQ范围分别为0.12 - 0.54 ng/mL和0.38-1.78 ng/mL。三个水平的重复性和准确度偏倚分别≤11%和10%以内。此外，基质效应范围为88%-118%，也符合欧洲药品管理局提供的生物分析方法验证指南。
In this work, the effect of sample matrix on electromembrane extraction (EME) was investigated for the first time using cathinones (log P < 1.0) as polar basic model analytes. Ten supported liquid membranes (SLMs) were tested for EME from spiked buffer solutions, urine, and whole blood samples, respectively. For buffer solutions, SLMs containing aromatic solvents provided higher EME recovery than non-aromatic solvents, which confirmed the significance of cation-π interactions for EME of basic substances. Interestingly, when applied to urine and whole blood samples, aromatic SLMs were less efficient, while non-aromatic SLMs containing abundant hydrogen-bond acidity/basicity were efficient. These observations were explained by SLM fouling, and the antifouling property of the SLM was clearly dependent on the nature of the SLM solvent. Accordingly, a binary SLM containing aromatic 1-ethyl-2-nitrobenzene (ENB) and non-aromatic 1-undecanol (1:1 v/v) was developed. This binary SLM was not prone to fouling, and provided high recoveries of cathinones from urine and whole blood. EME based on this SLM was optimized and evaluated in combination with liquid chromatography tandem mass spectrometry (LC-MS/MS), and the linear ranges with R2≥ 0.9903 for cathinones in whole blood and urine were 5–200 ng/mL and 1–200 ng/mL, respectively. The LOD and LOQ of cathinones were ranged from 0.12 to 0.54 ng/mL and 0.38–1.78 ng/mL, respectively. The repeatability and accuracy bias at three levels were ≤11% and within 10%, respectively. In addition, the matrix effect ranged from 88% to 118% was also in compliance with guidelines for bioanalytical method validation provided by the European Medicines Agency.