Cotranscriptional recruitment to the mRNA export receptor Mex67p contributes to nuclear pore anchoring of activated genes
Cotranscriptional recruitment to the mRNA export receptor Mex67p contributes to nuclear pore anchoring of activated genes
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DOI：
10.1128/mcb.00870-06
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发表时间：
2006-11-01
影响因子：
5.3
通讯作者：
Stutz, Francoise
中科院分区：
文献类型：
--
作者：
Dieppois, Guennaelle;Iglesias, Nahid;Stutz, Francoise
一些酿酒酵母基因的转录激活与它们重新定位到核外周是平行的，但基因锚定的机制尚不清楚。我们发现与核孔复合体相关的Mlp1p和穿梭mRNA输出受体Mex67p有助于激活的GAL10和Hsp104基因与核外周的稳定结合。然而，我们没有发现基因定位和基因表达之间的必然联系。此外，基因锚定与Mex67p对转录基因的共转录招募有关。值得注意的是，Mex67p与染色质的结合不是由RNA介导的。有趣的是，缺乏编码区的突变GAL2基因仍然能够在转录激活时招募Mex67p并重新定位到核外围。综上所述，这些数据表明，至少对GAL2来说，新生信使核糖核蛋白在基因锚定中不起主要作用，Mex67p的早期招募通过一个不依赖RNA的过程有助于基因重新定位。
Transcription activation of some Saccharomyces cerevisiae genes is paralleled by their repositioning to the nuclear periphery, but the mechanism underlying gene anchoring is poorly defined. We show that the nuclear pore complex-associated Mlp1p and the shuttling mRNA export receptor Mex67p contribute to the stable association of the activated GAL10 and HSP104 genes with the nuclear periphery. However, we find no obligatory link between gene positioning and gene expression. Furthermore, gene anchoring correlates with the cotranscriptional recruitment of Mex67p to transcribing genes. Notably, the association of Mex67p with chromatin is not mediated by RNA. Interestingly, a mutant GAL2 gene lacking the coding region is still able to recruit Mex67p upon transcriptional activation and to relocate to the nuclear periphery. Together these data suggest that, at least for GAL2, nascent messenger ribonucleoprotein does not play a major role in gene anchoring and that the early recruitment of Mex67p contributes to gene repositioning by virtue of an RNA-independent process.