PSEUDOMONAS-AERUGINOSA INFECTION IN CYSTIC-FIBROSIS - NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL REGULATION OF THE ALGD GENE
PSEUDOMONAS-AERUGINOSA INFECTION IN CYSTIC-FIBROSIS - NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL REGULATION OF THE ALGD GENE
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DOI：
10.1093/nar/15.11.4567
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发表时间：
1987-06-11
影响因子：
14.9
通讯作者：
CHAKRABARTY, AM
中科院分区：
文献类型：
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作者：
DERETIC, V;GILL, JF;CHAKRABARTY, AM
粘液、藻酸盐、铜绿假单胞菌引起的肺部感染是囊性纤维化患者的主要并发症。为了分析导致铜绿假单胞菌粘液样肺炎的机制，对编码GDP甘露糖脱氢酶的藻D基因进行了控制研究。藻类D的转录激活是藻酸盐生产所必需的。对algD及其启动子的测序显示，在转录起始上游和整个启动子区域都有多个直接重复序列。通过海藻D-XylE的转录融合，证实了海藻D启动子受海藻R基因的正调控。该基因此前已被证明经历了抗生素促进的染色体扩增，导致了粘液表型的出现。这些发现为更好地了解铜绿假单胞菌粘液样物质的控制提供了基础。
Pulmonary infection by mucoid, alginate producing, Pseudomonas aeruginosa is a major complication in patients suffering from cystic fibrosis (CF). To analyze the mechanisms leading to the emergence of mucoid P. aeruginosa in CF lungs, control of the algD gene coding for GDPmannose dehydrogenase was studied. Transcriptional activation of algD was shown to be necessary for alginate production. Sequencing of algD and its promoter revealed multiple direct repeats upstream of the transcription start and throughout the promoter region. Using the algD-xylE transcriptional fusion the algD promoter was demonstrated to be under positive control by the algR gene. This gene has previously been shown to undergo antibiotic promoted chromosomal amplification resulting in the emergence of the mucoid phenotype. These findings provide a basis for better understanding the control of mucoidy in P. aeruginosa.