Crystallization of protein-ligand complexes.
Crystallization of protein-ligand complexes.
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DOI：
10.1107/s0907444906047020
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发表时间：
2007-01
期刊：
影响因子：
--
通讯作者：
Shewchuk LM
中科院分区：
文献类型：
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作者：
Hassell AM;An G;Bledsoe RK;Bynum JM;Carter HL 3rd;Deng SJ;Gampe RT;Grisard TE;Madauss KP;Nolte RT;Rocque WJ;Wang L;Weaver KL;Williams SP;Wisely GB;Xu R;Shewchuk LM
提出的用于生长蛋白质-配体复合物的方法属于蛋白质与感兴趣的配体的共表达、在蛋白质纯化期间使用配体、共结晶和将配体浸泡到现有晶体中的类别。获得衍射质量的晶体长期以来一直是解决蛋白质三维结构的瓶颈。通常，当蛋白质与底物、核酸、辅因子或小分子复合时，它们可以被稳定化。另一方面，这些配体具有诱导蛋白质发生显著构象变化的潜力，并且可能需要从头筛选以找到新的晶体形式。本文概述了在以下领域获得蛋白质-配体复合物晶体的策略：（i）蛋白质与感兴趣的配体的共表达，（ii）在蛋白质纯化过程中使用配体，（iii）共结晶和（iv）浸泡。
Methods presented for growing protein–ligand complexes fall into the categories of co-expression of the protein with the ligands of interest, use of the ligands during protein purification, cocrystallization and soaking the ligands into existing crystals. Obtaining diffraction-quality crystals has long been a bottleneck in solving the three-dimensional structures of proteins. Often proteins may be stabilized when they are complexed with a substrate, nucleic acid, cofactor or small molecule. These ligands, on the other hand, have the potential to induce significant conformational changes to the protein and ab initio screening may be required to find a new crystal form. This paper presents an overview of strategies in the following areas for obtaining crystals of protein–ligand complexes: (i) co-expression of the protein with the ligands of interest, (ii) use of the ligands during protein purification, (iii) cocrystallization and (iv) soaks.