Characterization of BiP Genes from Pepper (Capsicum annuum L.) and the Role of CaBiP1 in Response to Endoplasmic Reticulum and Multiple Abiotic Stresses.
Characterization of BiP Genes from Pepper (Capsicum annuum L.) and the Role of CaBiP1 in Response to Endoplasmic Reticulum and Multiple Abiotic Stresses.
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辣椒 (Capsicum annuum L.) BiP 基因的特征以及 CaBiP1 在内质网和多种非生物胁迫响应中的作用
DOI：
10.3389/fpls.2017.01122
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发表时间：
2017
影响因子：
5.6
通讯作者：
Lu M
中科院分区：
文献类型：
--
作者：
Wang H;Niu H;Zhai Y;Lu M
不利的环境条件对作物的生长和发育具有不利影响，并导致蛋白质变性或错误折叠。结合蛋白（BiP）通过减轻错误折叠蛋白引起的内质网应激而发挥重要的保护作用。在这项研究中，我们的特点是三个BiP基因（CaBiP 1，CaBiP 2和CaBiP 3）在辣椒，一个经济上重要的蔬菜和香料物种。研究了CaBiP 1在植物对内质网胁迫和逆境（包括热、盐、渗透和干旱胁迫）的耐受性中的作用。所有预期的功能和信号结构域中检测到的三个BiP蛋白，但图案和外显子-内含子分布略有不同CaBiP 3。CaBiP 1和CaBiP 2在正常和应激条件下均在所有受试组织中组成型表达，而CaBiP 3主要在应激后表达。CaBiP 1基因的沉默降低了辣椒对内质网胁迫和各种环境胁迫的耐受性，并伴随着叶片中H2 O2积累、MDA含量、相对电导率（REL）、失水速率的增加以及可溶性蛋白含量和相对含水量（RWC）的降低。相反，在拟南芥中过表达CaBiP 1增强了对ER胁迫和多种环境胁迫的耐受性，表现为发芽率、根长、存活率、RWC、未折叠蛋白反应（UPR）途径的增加以及失水率的降低。我们的研究结果表明，CaBiP 1可能有助于植物耐受非生物胁迫，减少ROS积累，增加保水能力，刺激UPR途径和胁迫相关基因的表达。
Adverse environmental conditions have a detrimental impact on crop growth and development, and cause protein denaturation or misfolding. The binding protein (BiP) plays an important protective role by alleviating endoplasmic reticulum (ER) stress induced by misfolded proteins. In this study, we characterized three BiP genes (CaBiP1, CaBiP2, and CaBiP3) in pepper, an economically important vegetable and spice species. The role of CaBiP1 in plant tolerance to ER stress and adverse environmental conditions (including heat, salinity, osmotic and drought stress) were investigated. All the expected functional and signaling domains were detected in three BiP proteins, but the motifs and exon-intron distribution differed slightly in CaBiP3. CaBiP1 and CaBiP2 were constitutively expressed in all the tested tissues under both normal and stressed conditions, whereas CaBiP3 was mainly expressed following stress. Silencing of CaBiP1 reduced pepper tolerance to ER stress and various environment stresses, and was accompanied by increased H2O2 accumulation, MDA content, relative electric leakage (REL), water loss rate, and a reduction in soluble protein content and relative water content (RWC) in the leaves. Conversely, overexpression of CaBiP1 in Arabidopsis enhanced tolerance to ER stress and multiple environment stresses, as demonstrated by an increase in germination rate, root length, survival rate, RWC, the unfolded protein response (UPR) pathway, and a decrease in water loss rate. Our results suggest that CaBiP1 may contribute to plant tolerance to abiotic stresses by reducing ROS accumulation, increasing the water-retention ability, and stimulating UPR pathways and expression of stress-related genes.