ALTERATIONS OF GLUCOSE-TRANSPORTER MESSENGER-RNA AND PROTEIN-LEVELS IN BRAIN FOLLOWING THERMAL-INJURY AND SEPSIS IN MICE
ALTERATIONS OF GLUCOSE-TRANSPORTER MESSENGER-RNA AND PROTEIN-LEVELS IN BRAIN FOLLOWING THERMAL-INJURY AND SEPSIS IN MICE
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DOI：
10.1097/00024382-199406000-00001
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发表时间：
1994-06-01
期刊：
影响因子：
3.1
通讯作者：
HOFMANN, CA
中科院分区：
文献类型：
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作者：
GAMELLI, RL;LIU, H;HOFMANN, CA
由于葡萄糖的转运和利用深受损伤和感染的影响，而大脑是主要利用葡萄糖作为能量来源的器官，因此我们检查了热损伤和感染后大脑中易化葡萄糖转运蛋白 GLUT1 和 GLUT3 的状态。 BDF1 小鼠经历了 15% 全身表面积烧伤，有或没有铜绿假单胞菌感染。在损伤+/-感染后4和72小时，通过Northern印迹法测量GLUT1和GLUT3 mRNA丰度，并使用Western印迹法测定相关蛋白。 4小时时，烧伤（mRNA 150 +/- 12%，蛋白质122 +/- 6%）和烧伤/感染（mRNA 165 +/- 11%，蛋白质119 +/- 5%）动物的大脑GLUT1 mRNA和蛋白质丰度显着增加。 72小时时，烧伤（mRNA：139 +/- 11％，蛋白质：120 +/- 7％）和烧伤/感染（mRNA：145 +/- 14％，蛋白质：138 +/- 8％）动物中的GLUT1 mRNA和蛋白质水平也显着增加。我们的研究表明，GLUT1 mRNA 和蛋白质丰度的改变是对烧伤的主要反应，并且不会因烧伤创面感染而进一步改变。
Since glucose transport and utilization are profoundly influenced by injury and infection, and the brain is an organ which primarily utilizes glucose as its energy source, we examined the status of the facilitative glucose transporters GLUT1 and GLUT3 in brain following thermal injury and infection. BDF1 mice underwent a 15% total body surface area burn with or without Pseudomonas aeruginosa infection. At 4 and 72 h post injury +/- infection, GLUT1 and GLUT3 mRNA abundance was measured by Northern blotting, and the correlative proteins determined using Western blotting. At 4 h, both brain GLUT1 mRNA and protein abundance were significantly increased in burned (mRNA 150 +/- 12%, protein 122 +/- 6%) and burn/infected (mRNA 165 +/- 11%, protein 119 +/- 5%) animals. At 72 h, GLUT1 mRNA and protein levels were also significantly increased in burn (mRNA: 139 +/- 11%, protein: 120 +/- 7%) and burn/infected (mRNA: 145 +/- 14%, protein: 138 +/- 8%) animals. Our studies suggest that alterations of GLUT1 mRNA and protein abundance were primary responses to the burn injury and were not further altered by burn wound infection.