Flavonoid constituents and antioxidant capacity in flowers of different Zhongyuan tree penoy cultivars
Flavonoid constituents and antioxidant capacity in flowers of different Zhongyuan tree penoy cultivars
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DOI：
10.1016/j.jff.2011.09.006
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发表时间：
2012-01-01
影响因子：
5.6
通讯作者：
Tao, Guanjun
中科院分区：
文献类型：
--
作者：
Fan, Jinling;Zhu, Wenxue;Tao, Guanjun
采用高效液相色谱-光电二极管阵列检测-电喷雾电离质谱联用技术(HPLC-DAD-ESIMS)对48个中原牡丹品种花中的黄酮类成分进行了鉴定。采用 Folin-Ciocalteu 法测定总酚含量 (TPC)，并采用 2,2-二苯基-1-三硝基苯肼 (DPPH) 和铁还原抗氧化能力 (FRAP) 测定法测定抗氧化活性。共描述了约 14 种黄酮类化合物，包括 5 种花青素、3 种黄酮醇苷和 6 种黄酮苷。芹菜素己糖苷和芹菜素己糖苷酸为首次报道。 TPC（以没食子酸含量表示）在 3.97 至 21.73 毫克/克鲜重之间变化。不同品种的抗氧化活性各不相同（DPPH 和 FRAP 测定分别为 7.66-31.36 和 4.80-17.87 毫克抗坏血酸当量/克鲜重），并且与 TPC 高度一致。 (C) 2011 Elsevier Ltd. 保留所有权利。
Flavonoid components in flowers of 48 Zhongyuan tree penoy cultivars were identified by means of a hyphenated technique of HPLC coupled with a photodiode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESIMS). The total phenolic content (TPC) was determined by Folin-Ciocalteu method and the antioxidant activity with both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. About 14 flavonoids including five anthocyanins, three flavonol glycosides and six flavone glycosides were described. Apigenin-pento-hexoside and Apigenin-hexo-glucuronide were reported for the first time. TPC, expressed as gallic acid content, varied between 3.97 and 21.73 mg/g fresh weight. Antioxidant activity varied among the cultivars (7.66-31.36 and 4.80-17.87 mg ascorbic acid equivalents/g fresh weight for DPPH and FRAP assays, respectively) and highly corresponded with TPC. (C) 2011 Elsevier Ltd. All rights reserved.