Functional dissection of a rice high-pI alpha-amylase gene promoter.
Functional dissection of a rice high-pI alpha-amylase gene promoter.
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水稻高 pI α-淀粉酶基因启动子的功能剖析。
DOI：
10.1007/bf00283513
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Wu,R
中科院分区：
文献类型：
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作者：
Tanida,I;Kim,JK;Wu,R
先前的缺失分析表明，位于水稻高pI α-淀粉酶基因OSamy-c 5′端-230和+29位置之间的260 bp片段是赤霉酸（GA 3）依赖性转录激活所必需的。我们已经建立了一个基于荧光素酶报告基因在水稻糊粉细胞中表达的定量瞬时测定方法，并继续表征GA 3依赖性调控序列的OSamy-c启动子。使用这种方法，我们已经证明了-158和-46之间的DNA序列（序列I）足以赋予OSamy-c GA 3响应性激活。我们还表明，该序列是能够指导GA 3依赖性表达从异源最小启动子。我们的研究结果还表明，序列I赋予GA 3的调节控制在一个方向依赖的方式和相互作用的两个进一步的上游DNA序列，II和III，在一个组合，这温和地提高了GA 3的响应水平的序列I所展示的。因此，我们建议，序列I赋予的基本GA 3响应字符onOSamy-c，并结合序列II和III的调节蛋白相互作用，并与结合序列I的调节蛋白，有效地调节GA 3响应。
Deletion analysis has previously shown that a 260 by fragment, located between positions −230 and +29 of the 5′ end of a rice high-pI α-amylase gene,OSamy-c, is required for gibberellic acid (GA3)-dependent transcriptional activation. We have since established a quantitative transient assay based on expression of a luciferase reporter gene in rice aleurone cells and continued to characterize theOSamy-cpromoter for GA3-dependent regulatory sequences. Using this method, we have shown that the DNA sequence between−158 and −46 (sequence I) is sufficient to confer GA3-responsive activation onOSamy-c. We have also shown that this sequence is capable of directing GA3-dependent expression from a heterologous minimal promoter. Our results also showed that sequence I confers GA3regulatory control in an orientation-dependent manner and interacts with two further upstream DNA sequences, II and III, in a combination which mildly enhances the level of the GA3response exhibited by sequence I. Thus, we propose that sequence I confers the fundamental GA3-responsive character onOSamy-c, and that regulatory proteins that bind sequences II and III interact with each other and with regulatory proteins that bind sequence I, effectively to modulate the GA3response.