Alkaloid purification using rosin-based polymer-bonded silica stationary phase in HPLC
Alkaloid purification using rosin-based polymer-bonded silica stationary phase in HPLC
复制标题
在 HPLC 中使用松香基聚合物键合硅胶固定相纯化生物碱
DOI：
10.1002/jssc.201900835
复制
发表时间：
2019-10-23
影响因子：
3.1
通讯作者：
Lei, Fuhou
中科院分区：
文献类型：
--
作者：
Sun, Yao;Li, Pengfei;Lei, Fuhou
生物碱是重要的天然产物，具有广泛的药理活性。为了有效地分离和提纯它们，通过乙二醇马来酸酯、丙烯酸酯和甲基丙烯酸在功能化硅胶上的表面自由基聚合，合成了松香基聚合物键合硅胶高效液相色谱固定相。对固定相、色谱柱、生物碱色谱条件的优化以及分析物在色谱柱上的热力学行为进行了全面的研究。在优化的条件下，所制备的色谱柱可有效纯化天然喜树碱、咖啡因和吴茱萸碱，纯度分别为92%、96%和97%。通过这项工作，我们开发了一种有效的分离生物碱的方法，促进了松香基材料在生物医学和分析化学中的研究。
Alkaloids are important natural products that exhibit a wide spectrum of pharmacological activities. To efficiently separate and purify them, a rosin-based polymer-bonded silica stationary phase in high-performance liquid chromatography was synthesized via the surface radical polymerization of ethylene glycol maleic rosinate acrylate and methacrylic acid onto functionalized silica. The stationary phases, columns, optimization of chromatographic conditions for alkaloids, and thermodynamic behavior of the analytes on the column were fully studied. Under the optimized conditions, the prepared column efficiently purified natural camptothecine, caffeine, and evodiamine with the corresponding purities of 92, 96, and 97%. With this work, we have developed an efficient approach to isolate alkaloids and promoted the research on rosin-based materials in biomedicine and analytical chemistry.