Quadrupole Time-of-Flight Mass Spectrometry: A Paradigm Shift in Toxicology Screening Applications.
Quadrupole Time-of-Flight Mass Spectrometry: A Paradigm Shift in Toxicology Screening Applications.
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DOI：
10.33176/aacb-19-00023
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发表时间：
2019-08-01
期刊：
影响因子：
--
通讯作者：
McWhinney, Brett C
中科院分区：
文献类型：
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作者：
Allen, Darren R;McWhinney, Brett C
筛选生物样本中是否存在非法或法律的物质是临床和法医毒理学的重要前沿工具。在临床环境中，药物筛选是临床医生改善患者护理和指导治疗的有用工具。用于筛选生物样品中的药物的分析方法是广泛的并且有据可查的，尽管许多快速筛选技术通常缺乏适当的灵敏度和特异性，需要仔细的临床解释。新型精神活性物质的不断出现给保持最新的相关药物检测方法带来了相当大的分析挑战。调整和验证用于检测新物质的方法可能是一项复杂而昂贵的工作。新药的出现和商业检测方法的发布之间也有相当长的滞后时间。四极杆飞行时间质谱（Q-TOF-MS）作为一种能够应对快速变化的药物环境的挑战的分析技术在过去十年中获得了相当大的关注。Q-TOF-MS在药物检测中表现出高灵敏度和特异性，还允许快速更新新出现的精神活性剂的方法。Q-TOF-MS与液相或气相色谱等技术的结合可以提供快速和全面的筛查解决方案，这些解决方案在临床实验室环境中越来越受欢迎。
The screening of biological samples for the presence of illicit or legal substances is an important frontline tool in both clinical and forensic toxicology. In the clinical setting, drug screening is a useful tool for the clinician in improving patient care and guiding treatment. Analytical approaches for the screening of drugs in biological samples are extensive and well documented, though many rapid screening techniques often lack appropriate sensitivity and specificity, requiring careful clinical interpretation. The continuous emergence of new psychoactive substances presents a considerable analytical challenge in maintaining up-to-date methods for the detection of relevant drugs. Adapting and validating methods for the detection of new substances can be a complicated and costly undertaking. There is also a considerable lag time between the emergence of new drugs and the release of commercial assays for detection. Quadrupole time-of-flight mass spectrometry (Q-TOF-MS) has gained considerable attention over the last decade as an analytical technique that is capable of meeting the challenges of a rapidly changing drug landscape. Exhibiting both high sensitivity and specificity in drug detection, Q-TOF-MS also allows methods to be rapidly updated for newly emerging psychoactive agents. The coupling of Q-TOF-MS with techniques such as liquid or gas chromatography can provide both rapid and comprehensive screening solutions that are gaining popularity in the clinical laboratory setting.