NMR study of isoleucine transfer RNA from Thermus thermophilus.
NMR study of isoleucine transfer RNA from Thermus thermophilus.
复制标题
嗜热栖热菌异亮氨酸转移 RNA 的 NMR 研究。
DOI：
10.1021/bi00355a010
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Redfield,AG
中科院分区：
文献类型：
--
作者：
Choi,BS;Redfield,AG
Department of Biochemistry, Brandéis University, Waltham, Massachusetts 02254 Received June 10, 1985; Revised Manuscript Received October 1, 1985 abstract: An NMR and nuclear Overhauser effect (NOE) analysis of Thermus thermophilus tRNA1/® is presented. This species contains modifications including s2* T54 and s4U8 [Horie, N., Hara-Yokoyama, M., Yokoyama, S., Watanabe, K., Kuchino, Y., Nishimura, S., & Miyazawa, T.(1985) Biochemistry 24, 5711-5715]. All theexpected secondary and reverse Hoogsteen AU pairs were identified, with one possible exception. The general geometry of the loop is the same as the Escherichia coli species, andthere is NOE evidence for an A9-UA12 triple. Preliminary measurements of solvent exchange rates of internally hydrogen-bonded bases suggest that this tRNA is more stable than previously studied E. coli and yeast tRNAs.
