Gabaergic neurones in the rat periaqueductal grey matter express α4, β1 and δ GABAA receptor subunits:: Plasticity of expression during the estrous cycle
Gabaergic neurones in the rat periaqueductal grey matter express α4, β1 and δ GABAA receptor subunits:: Plasticity of expression during the estrous cycle
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DOI：
10.1016/j.neuroscience.2005.08.013
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Lovick, TA
中科院分区：
文献类型：
--
作者：
Griffiths, JL;Lovick, TA
本文观察了Wistar大鼠动情周期不同阶段中脑导水管周围灰质神经元上GABA（A）受体α 4、β 1和δ亚单位的免疫反应性。α 4、β 1和δ GABA（A）受体亚单位的免疫染色存在于整个导水管周围灰质的神经元上。亚单位免疫反应神经元的数量保持不变，在发情周期的早期阶段（发情前期早期间情期），但在后期间情期显着增加。GABA合成酶谷氨酸脱羧酶的双重免疫标记显示，几乎90%的亚基阳性细胞含有谷氨酸脱羧酶的免疫反应性。在发情周期的早期阶段（发情前期至间情期早期），大约三分之一的谷氨酸脱羧酶阳性群体共定位α 4、β 1和δ GABA（A）受体亚单位。在动情间期后期，当亚基阳性细胞的数量增加时，表达α 4的含谷氨酸脱羧酶的群体的比例，β 1和δ GABA（A）受体亚单位几乎增加了一倍。具有α 4 β 1 δ构型的受体由导水管周围灰质中的GABA能神经元表达，并且表达这些亚单位的细胞数量增加与血浆孕酮水平下降一致。GABA（A）受体表达的变化可能导致导水管周围灰质神经回路兴奋性的变化。(c)2005年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Immunoreactivity for alpha 4, beta 1 and delta GABA(A) receptor subunits on neurones in the periaqueductal gray matter was investigated at different stages of the estrous cycle in Wistar rats. Immunostaining for alpha 4, beta 1 and delta GABA(A) receptor subunits was present on neurones throughout the periaqueductal gray matter. The numbers of subunit-immunoreactive neurones remained constant during the early phases of the estrous cycle (proestrus to early diestrus) but increased significantly in late diestrus. Dual immunolabeling for the GABA synthesizing enzyme glutamic acid decarboxylase revealed that almost 90% of the subunit-positive cells contained immunoreactivity for glutamic acid decarboxylase. During the early phases of the estrous cycle (proestrus to early diestrus), approximately one third of the glutamic acid decarboxylase-positive population co-localized alpha 4, beta 1 and delta GABA(A) receptor subunits. When the number of subunit positive cells increased during late diestrus, the proportion of the glutamic acid decarboxylase-containing population that expressed alpha 4, beta 1 and delta GABA(A) receptor subunits almost doubled.We propose that GABA(A) receptors with the alpha 4 beta 1 delta configuration are expressed by GABAergic neurones in the periaqueductal gray matter and that the numbers of cells expressing these subunits are increased in late diestrus in line with failing plasma progesterone levels. Changes in GABA(A) receptor expression may lead to changes in the excitability of the neural circuitry in the periaqueductal gray matter. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.