THE POP1 GENE ENCODES PROTEIN-COMPONENT COMMON TO THE RNASE-MRP AND RNASE-P RIBONUCLEOPROTEINS
THE POP1 GENE ENCODES PROTEIN-COMPONENT COMMON TO THE RNASE-MRP AND RNASE-P RIBONUCLEOPROTEINS
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DOI：
10.1101/gad.8.12.1423
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发表时间：
1994-06-15
影响因子：
10.5
通讯作者：
TOLLERVEY, D
中科院分区：
文献类型：
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作者：
LYGEROU, Z;MITCHELL, P;TOLLERVEY, D
两种形式的酵母5.88 rRNA通过不同的加工途径从一个大的前体产生。A3位点的裂解是合成主要的短形式（命名为5.8S（S））所必需的，但不是合成长形式5.8S（L）所必需的。为了鉴定A3裂解所需的组分，筛选了温度敏感性致死突变体库中具有5.8S（S）：5.8S（L）降低比率的那些。pop 1 -1突变（用于前体RNA的加工）显示了这种表型，也抑制了A3切割。前rRNA加工缺陷的pop 1 -1菌株是类似于报告的突变的RNA酶MRP的RNA组分中，我们表明，在RNase MRP RNA的突变也抑制切割位点A3。这是第一个在体内需要RNase MRP切割的位点。Pop 1 -1突变也导致前tRNA的加工中的一个块，这与RNase P的RNA组分中的突变所报道的相同。RNase MRP和RNase P的RNA组分在非允许温度下在Pop 1 -1菌株中积累不足，免疫沉淀表明POP 1 p是两种核糖核蛋白的组分。POP 1基因编码的蛋白质的预测分子量为100.5 kD，是必不可少的活力。POP 1 p是核RNase P或RNase MRP的第一个蛋白质组分，其基因已被克隆。
Two forms of the yeast 5.88 rRNA are generated from a large precursor by distinct processing pathways. Cleavage at site A3 is required for synthesis of the major, short form, designated 5.8S(S), but not for synthesis of the long form, 5.8S(L). To identify components required for A3 cleavage, a bank of temperature-sensitive lethal mutants was screened for those with a reduced ratio of 5.8S(S):5.8S(L). The pop1-1 mutation (for processing of precursor RNAs) shows this phenotype and also inhibits A3 cleavage. The pre-rRNA processing defect of pop1-1 strains is similar to that reported for mutations in the RNA component of RNase MRP; we show that a mutation in the RNase MRP RNA also inhibits cleavage at site A3. This is the first site shown to require RNase MRP for cleavage in vivo. The pop1-1 mutation also leads to a block in the processing of pre-tRNA that is identical to that reported for mutations in the RNA component of RNase P. The RNA components of both RNase MRP and RNase P are underaccumulated in pop1-1 strains at the nonpermissive temperature, and immunoprecipitation demonstrates that POP1p is a component of both ribonucleoproteins. The POP1 gene encodes a protein with a predicted molecular mass of 100.5 kD and is essential for viability. POP1p is the first protein component of the nuclear RNase P or RNase MRP for which the gene has been cloned.