Identification of putative Arabidopsis DEMETER target genes by GeneChip analysis.
Identification of putative Arabidopsis DEMETER target genes by GeneChip analysis.
复制标题
通过基因芯片分析鉴定假定的拟南芥 DEMETER 靶基因。
DOI：
10.1016/j.bbrc.2007.10.092
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发表时间：
2007
影响因子：
3.1
通讯作者：
Choi,Yeonhee
中科院分区：
文献类型：
--
作者：
Ohr,Hyonhwa;Bui,AnhthuQ;Le,BrandonH;Fischer,RobertL;Choi,Yeonhee
在胚乳中，印迹聚梳组(Medea和FIS2)和转录因子(FWA)基因的母体等位基因表达需要拟南芥DME(DME)DNA糖基酶。DME在中央细胞中的表达，而不是在花粉或雄蕊中的表达，通过降低母体等位基因的甲基化来建立基因印记。然而，人们对受DME调控的其他基因知之甚少。为了鉴定可能的DME靶基因，我们构建了在花粉和雄蕊中异位表达DME的CaMV：DME植株。通过比较，发现94个基因是由异位表达的DME在雄蕊和花粉中诱导的。基因本体论分析确定了在DME诱导的RNA列表中丰富的三种分子功能：DNA或RNA结合、激酶活性和转录因子活性。半定量RT-PCR验证了基因芯片分析确定的候选基因。本研究中确定的可能的靶基因将为深入了解DME DNA糖基酶的调控机制和DNA去甲基化的功能提供依据。
The Arabidopsis DEMETER (DME) DNA glycosylase is required for the maternal allele expression of imprinted Polycomb group (MEDEA and FIS2) and transcription factor (FWA) genes in the endosperm. Expression of DME in the central cell, not in pollen or stamen, establishes gene imprinting by hypomethylating maternal alleles. However, little is known about other genes regulated by DME. To identify putative DME target genes, we generated CaMV:DME plants which ectopically express DME in pollen and stamens. Comparison of mRNA profiles revealed 94 genes induced by ectopic DME expression in both stamen and pollen. Gene ontology analysis identified three molecular functions enriched in the DME-inducible RNA list: DNA or RNA binding, kinase activity, and transcription factor activity. Semi-quantitative RT-PCR verified the candidate genes identified by GeneChip analysis. The putative target genes identified in this study will provide insights into the regulatory mechanism of DME DNA glycosylase and the functions of DNA demethylation.