Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy
Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy
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DOI：
10.1117/1.3147385
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发表时间：
2009-05-01
影响因子：
3.5
通讯作者：
Marquet, Pierre
中科院分区：
文献类型：
--
作者：
Rappaz, Benjamin;Cano, Elena;Marquet, Pierre
数字全息显微术（DHM）是一种光学技术，它提供的相位图像产生定量信息的细胞结构和细胞动力学。此外，定量相位图像允许导出其他参数，包括干批量生产、密度和空间分布。我们应用DHM研究了野生型和突变型裂殖酵母细胞的干物质生产速率和干物质表面密度。我们的研究证明了DHM作为细胞周期无标记定量分析工具的适用性，并为其在高通量筛选中的应用开辟了可能性。(C)2009年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI 10.1117/1.3147385]
Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3147385]