Ultrathin polymeric coatings based on hydrogen-bonded polyphenol for protection of pancreatic islet cells.
Ultrathin polymeric coatings based on hydrogen-bonded polyphenol for protection of pancreatic islet cells.
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DOI：
10.1002/adfm.201200138
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发表时间：
2012-08-21
影响因子：
19
通讯作者：
Kharlampieva, Eugenia
中科院分区：
文献类型：
--
作者：
Kozlovskaya, Veronika;Zavgorodnya, Oleksandra;Chen, Yi;Ellis, Kristin;Tse, Hubert M.;Cui, Wanxing;Thompson, J. Anthony;Kharlampieva, Eugenia
尽管胰岛细胞移植已成为治疗1型糖尿病的一种很有前景的方法，但由于包括致病性先天免疫反应和适应性免疫反应在内的许多限制，其临床应用仍然有限。我们报道了一种新型的多功能细胞保护材料，用于覆盖活胰岛。该涂层利用天然多酚（单宁酸）与聚（n-乙烯基吡咯烷酮）的氢键相互作用，通过非离子逐层组装沉积在胰岛表面。我们证明了该涂层在哺乳动物胰岛表面是保形的，包括来自大鼠，非人灵长类动物（NHP）和人类的胰岛。与未经修饰的对照组相比，包被的胰岛在体外至少能保持96小时的活力和β细胞功能。我们还确定涂层具有免疫调节细胞保护特性，可抑制受刺激的骨髓源性巨噬细胞和糖尿病源性BDC-2.5 T细胞中促炎细胞因子的合成。该涂层材料在生理相关条件下具有较高的化学稳定性，并具有抑制细胞因子合成的能力，这是延长胰岛完整性、活力和体内功能的关键参数。我们的研究为用于细胞移植治疗的先进多功能材料领域提供了新的机会。
Though transplantation of pancreatic islet cells has emerged as a promising treatment for Type 1 diabetes its clinical application remains limited due to a number of limitations including both pathogenic innate and adaptive immune responses. We report here on a novel type of multifunctional cytoprotective material applied to coat living pancreatic islets. The coating utilizes hydrogen-bonded interactions of a natural polyphenol (tannic acid) with poly(N-vinylpyrrolidone) deposited on the islet surface via non-ionic layer-by-layer assembly. We demonstrate that the coating is conformal over the surface of mammalian islets including those derived from rat, non-human primate (NHP), and human. In contrast to unmodified controls, the coated islets maintain their viability and β-cell functionality for at least 96 hours in vitro. We also determine that the coating demonstrates immunomodulatory cytoprotective properties suppressing pro-inflammatory cytokine synthesis in stimulated bone marrow-derived macrophages and diabetogenic BDC-2.5 T cells. The coating material combines high chemical stability under physiologically relevant conditions with capability of suppressing cytokine synthesis, crucial parameters for prolonged islet integrity, viability, and function in vivo. Our study offers new opportunities in the area of advanced multifunctional materials to be used for a cell-based transplantation therapy.
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影响因子：
4.4
作者：
Cantor, Joseph;Haskins, Kathryn
通讯作者：
Haskins, Kathryn
影响因子：
3.8
作者：
Contreras, JL;Xie, D;Eckhoff, DE
通讯作者：
Eckhoff, DE
影响因子：
19
作者：
Cheung, Charles Y.;McCartney, Suzanne J.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
DOI：
10.1084/jem.181.5.1755
发表时间：
1995-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Jacobson NG;Szabo SJ;Weber-Nordt RM;Zhong Z;Schreiber RD;Darnell JE Jr;Murphy KM
通讯作者：
Murphy KM
影响因子：
0.9
作者：
Abalovich, A;Jatimliansky, C;Nacucchio, M
通讯作者：
Nacucchio, M