Imaging of goblet cells as a marker for intestinal metaplasia of the stomach by one-photon and two-photon fluorescence endomicroscopy
Imaging of goblet cells as a marker for intestinal metaplasia of the stomach by one-photon and two-photon fluorescence endomicroscopy
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DOI：
10.1117/1.3269681
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发表时间：
2009-11-01
影响因子：
3.5
通讯作者：
Gu, Min
中科院分区：
文献类型：
--
作者：
Bao, Hongchun;Boussioutas, Alex;Gu, Min
杯状细胞是诊断胃肠化生的必要条件。胃粘膜由单层柱状上皮排列，隐窝处有一些特化，但正常胃上皮中没有杯状细胞。胃上皮中杯状细胞的出现是向腺癌的潜在恶性进展的指标。因此，体内杯状细胞的三维成像对于诊断被称为肠化生的胃癌癌前阶段是必不可少的。我们用含有杯状细胞的小鼠肠道作为肠化生的模型。采用单光子共聚焦荧光内窥镜和双光子荧光内窥镜对杯状细胞进行三维成像。演示了成像的穿透深度、切片能力和光漂白信息。两种内窥镜技术均可对小鼠大肠杯状细胞进行三维观察，成像深度达176微米，其中双光子荧光内窥镜显示出较高的分辨率和对比度。此外，双光子荧光内窥镜还具有切片能力强、光漂白少等优点。这些结果证明双光子荧光内窥镜在胃癌癌前阶段的诊断中是有优势的。(C)2009年，光学仪器工程师学会。[DOI：10.1117/1.3269681]
Goblet cells are a requirement for the diagnosis of intestinal metaplasia of the stomach. The gastric mucosa is lined by a monolayer of columnar epithelium with some specialization at the crypts, but there are no goblet cells in normal gastric epithelium. The appearance of goblet cells in gastric epithelium is an indicator of potential malignant progression toward adenocarcinoma. Therefore, in vivo three-dimensional imaging of goblet cells is essential for diagnoses of a premalignant stage of gastric cancers called intestinal metaplasia. We used mouse intestine, which has goblet cells, as a model of intestinal metaplasia. One-photon confocal fluorescence endomicroscopy and two-photon fluorescence endomicroscopy are employed for 3-D imaging of goblet cells. The penetration depth, the sectioning ability, and the photobleaching information of imaging are demonstrated. Both endomicroscopy techniques can three-dimensionally observe goblet cells in mouse large intestine and achieve an imaging depth of 176 mu m. The two-photon fluorescence endomicroscopy shows higher resolution and contrast of the imaging sections at each depth. In addition, two-photon fluorescence endomicroscopy also has advantages of sectioning ability and less photobleaching. These results prove that two-photon fluorescence endomicroscopy is advantageous in diagnoses of a premalignant stage of gastric cancers. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3269681]