A Genetic Screen Identifies a Requirement for Cysteine-Rich-Receptor-Like Kinases in Rice NH1 (OsNPR1)-Mediated Immunity.
A Genetic Screen Identifies a Requirement for Cysteine-Rich-Receptor-Like Kinases in Rice NH1 (OsNPR1)-Mediated Immunity.
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DOI：
10.1371/journal.pgen.1006049
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发表时间：
2016-05
期刊：
影响因子：
4.5
通讯作者：
Ronald PC
中科院分区：
文献类型：
--
作者：
Chern M;Xu Q;Bart RS;Bai W;Ruan D;Sze-To WH;Canlas PE;Jain R;Chen X;Ronald PC
系统获得性抗性由拟南芥NPR 1基因和水稻NH 1基因介导，赋予对多种病原体的广谱免疫。NPR 1和NH 1与TGA转录因子相互作用以激活下游防御基因。尽管这种防御反应的重要性，NPR 1/NH 1和TGA蛋白下游的信号传导组分的定义很差。在这里，我们报告的鉴定水稻突变体，snim 1，抑制NH 1介导的免疫，并证明两个基因编码以前未知的富含半胱氨酸的受体样激酶（CRK 6和CRK 10），补充snim 1突变表型。CRK 6和CRK 10基因在亲本遗传背景中单独沉默重建了snim 1表型。我们在Kitaake遗传背景中发现了一个crk 10移码突变的水稻突变体;该突变体还显示出一种受损的免疫反应，突出了crk 10的重要作用。我们还表明，NH 1表达水平的升高导致CRK 10表达增强，并且水稻TGA2.1蛋白与CRK 10启动子结合。这些实验证明了在NH 1介导的免疫中需要CRKs，并建立了NH 1和CRK 10表达诱导之间的分子联系。为了生存，植物和动物必须抵抗微生物的感染。植物采用称为系统获得性抗性的免疫反应，赋予对广谱病原体的持久抗性。研究人员先前已经确定了控制这种免疫反应的两种关键蛋白质（NPR 1/NH 1和TGA）。尽管取得了这些进展，但我们对这一重要免疫反应的认识和理解仍存在许多差距。我们已经确定了这种免疫反应所需的新基因（CRK 10）;没有它，植物更容易受到感染。这些发现推进了系统获得性抗性的基础知识，并为这一令人兴奋和重要的抗性机制的研究开辟了新的途径。
Systemic acquired resistance, mediated by the Arabidopsis NPR1 gene and the rice NH1 gene, confers broad-spectrum immunity to diverse pathogens. NPR1 and NH1 interact with TGA transcription factors to activate downstream defense genes. Despite the importance of this defense response, the signaling components downstream of NPR1/NH1 and TGA proteins are poorly defined. Here we report the identification of a rice mutant, snim1, which suppresses NH1-mediated immunity and demonstrate that two genes encoding previously uncharacterized cysteine-rich-receptor-like kinases (CRK6 and CRK10), complement the snim1 mutant phenotype. Silencing of CRK6 and CRK10 genes individually in the parental genetic background recreates the snim1 phenotype. We identified a rice mutant in the Kitaake genetic background with a frameshift mutation in crk10; this mutant also displays a compromised immune response highlighting the important role of crk10. We also show that elevated levels of NH1 expression lead to enhanced CRK10 expression and that the rice TGA2.1 protein binds to the CRK10 promoter. These experiments demonstrate a requirement for CRKs in NH1-mediated immunity and establish a molecular link between NH1 and induction of CRK10 expression. To survive, plants and animals must resist microbial infection. Plants employ an immune response called systemic acquired resistance that confers long-lasting resistance to a broad-spectrum of pathogens. Researchers have previously identified two key proteins (NPR1/NH1 and TGA) that control this immune response. Despite these advances, there remain many gaps in our knowledge and understanding of this important immune response. We have identified a new gene (CRK10) required for this immune response; without it, plants are more susceptible to infection. These findings advance basic knowledge of systemic acquired resistance and open the door to a new avenue of research on this exciting and important resistance mechanism.